Anti-MGAT5 抗体 [3E9] (ab87977)
![Western blot - Anti-MGAT5 antibody [3E9] (ab87977)](https://www.abcam.co.jp/ps/products/87/ab87977/Images/ab87977-254307-anti-mgat5-antibody-western-blot.jpg "Western blot - Anti-MGAT5 antibody [3E9] (ab87977)")
Key features and details
- Mouse monoclonal [3E9] to MGAT5
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
製品の概要
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製品名
Anti-MGAT5 antibody [3E9]
MGAT5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3E9] to MGAT5 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse, Rat, Hamster
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免疫原
Recombinant fragment: LAEPGQSCKQ VCQESQLICE PSFFQHLNKD KDMLKYKVTC QSSELAKDIL VPSFDPKNK HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKD, corresponding to amino acids 642 - 739 of Human MGAT5 (NP_002401) with a proprietary tag
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ポジティブ･コントロール
- Jurkat whole cell lysate (ab7899)
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特記事項
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
3E9 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87977の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa. |
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Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 18 family. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4249 Human
- Entrez Gene: 107895 Mouse
- Entrez Gene: 65271 Rat
- Omim: 601774 Human
- SwissProt: Q09328 Human
- SwissProt: Q8R4G6 Mouse
- SwissProt: Q08834 Rat
- Unigene: 651869 Human
see all -
別名
- 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
- 6-N-acetylglucosaminyltransferase antibody
- Alpha 1,3(6) mannosylglycoprotein antibody
see all
画像
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Western blot - Anti-MGAT5 antibody [3E9] (ab87977)Image is courtesy of an AbReview submitted by Armen Petrosyan.Anti-MGAT5 antibody [3E9] (ab87977) at 1/2000 dilution + Human RWPE prostate cells at 20 µg
Secondary
Donkey anti-mouse polyclonal HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Additional bands at: 55 kDa (possible non-specific binding), 95 kDa (possible post-translational modification)
Exposure time: 40 secondsBlocking was via 5% milk in PBST incubated for 1 hour at 22°C
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-MGAT5 antibody [3E9] (ab87977)](https://www.abcam.co.jp/ps/products/87/ab87977/Images/ab87977-208175-anti-mgat5-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-MGAT5 antibody [3E9] (ab87977)Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-MGAT5 antibody [3E9] (ab87977)](https://www.abcam.co.jp/ps/products/87/ab87977/Images/ab87977-102888-anti-mgat5-antibody-western-blot.jpg)
Western blot - Anti-MGAT5 antibody [3E9] (ab87977)Anti-MGAT5 antibody [3E9] (ab87977) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 85 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?The recombinant protein used as the immunogen has a predicted size of 37 kDa.
This image was generated using the ascites version of the product.
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![Western blot - Anti-MGAT5 antibody [3E9] (ab87977)](https://www.abcam.co.jp/ps/products/87/ab87977/Images/ab87977-102887-anti-mgat5-antibody-western-blot.jpg)
Western blot - Anti-MGAT5 antibody [3E9] (ab87977)Anti-MGAT5 antibody [3E9] (ab87977) at 1 µg/ml + jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 85 kDaThis image was generated using the ascites version of the product.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab87977 は 6 報の論文で使用されています。
- Kamiya T et al. Inhibition of N-glycosylation by glucosamine hydrochloride inhibits TGF-β1-induced LOXL2 secretion. J Cell Biochem 124:797-807 (2023). PubMed: 37062903
- Wang Y et al. RUNX2 promotes gastric cancer progression through the transcriptional activation of MGAT5 and MMP13. Front Oncol 13:1133476 (2023). PubMed: 37256183
- Cheng CC et al. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation. J Cell Physiol 237:1780-1789 (2022). PubMed: 34806177
- Cong X et al. Silencing GnT-V reduces oxaliplatin chemosensitivity in human colorectal cancer cells through N-glycan alteration of organic cation transporter member 2. Exp Ther Med 21:128 (2021). PubMed: 33376510
- Yan G et al. Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5. Am J Cancer Res 9:585-596 (2019). PubMed: 30949412
- Wang G et al. Loss of Barx1 promotes hepatocellular carcinoma metastasis through up-regulating MGAT5 and MMP9 expression and indicates poor prognosis. Oncotarget 8:71867-71880 (2017). PubMed: 29069753
