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AB324877

Anti-METTL2 antibody [EPR26701-205]

Anti-METTL2 antibody [EPR26701-205]

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Rabbit Recombinant Monoclonal METL2 antibody. Suitable for IP, WB and reacts with Mouse samples.

別名を表示する

D11Ertd768e, tRNA N(3)-cytidine methyltransferase METTL2, Methyltransferase-like protein 2

4 Images
Immunoprecipitation - Anti-METTL2 antibody [EPR26701-205] (AB324877)
  • IP

Supplier Data

Immunoprecipitation - Anti-METTL2 antibody [EPR26701-205] (AB324877)

METTL2 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab324877 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324877 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 2:

ab324877 at 1/30 IP in Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab324877 in Neuro-2 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 44 kDa

false

Exposure time: 180s

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)
  • WB

Supplier Data

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Neuro-2a transfected with siRNA specifically targeting METTL2 whole cell lysate at 20 µg

Lane 3:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)
  • WB

Supplier Data

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324877 was shown to bind specifically to METTL2. Target of interest was observed at 44 kDa in wild-type mouse tissues lysates (lanes 1, 3, 5, 7) with no signal observed at this size in METTL2 knockout tissues (lanes 2, 4, 6, 8).

The lysates were kindly provided by Dr Jiaqiang Zhou's lab, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Wild-type mouse liver tissue lysate at 20 µg

Lane 2:

METTL2 knockout mouse liver tissue lysate at 20 µg

Lane 3:

Wild-type mouse brain tissue lysate at 20 µg

Lane 4:

METTL2 knockout mouse brain tissue lysate at 20 µg

Lane 5:

Wild-type mouse kidney tissue lysate at 20 µg

Lane 6:

METTL2 knockout mouse kidney tissue lysate at 20 µg

Lane 7:

Wild-type mouse skeletal muscle tissue lysate at 20 µg

Lane 8:

METTL2 knockout mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)
  • WB

Supplier Data

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324877 was shown to bind specifically to METTL2. Target of interest was observed at 44 kDa in wild-type mouse tissues lysates (lanes 1, 3, 5) with no signal observed at this size in METTL2 knockout tissues (lanes 2, 4, 6).

The lysates were kindly provided by Dr Jiaqiang Zhou's lab, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Wild-type mouse subcutaneous adipose tissue lysate at 20 µg

Lane 2:

METTL2 knockout mouse subcutaneous adipose tissue lysate at 20 µg

Lane 3:

Wild-type mouse white adipose tissue lysate at 20 µg

Lane 4:

METTL2 knockout mouse white adipose tissue lysate at 20 µg

Lane 5:

Wild-type mouse brown adipose tissue lysate at 20 µg

Lane 6:

METTL2 knockout mouse brown adipose tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR26701-205

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr)(UGU) and tRNA(Arg)(CCU) (PubMed : 28655767). N(3)-methylcytidine methylation by METTL2 requires the N6-threonylcarbamoylation of tRNA (t6A37) by the EKC/KEOPS complex as prerequisite (By similarity).
See full target information Mettl2
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Abcam product promise

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