Anti-METTL14 抗体 [EPR22666-22] (ab300104)
![Western blot - Anti-METTL14 antibody [EPR22666-22] (AB300104)](https://www.abcam.co.jp/ps/products/300/ab300104/Images/AB300104-485989-WB2.jpg "Western blot - Anti-METTL14 antibody [EPR22666-22] (AB300104)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22666-22] to METTL14
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-METTL14 antibody [EPR22666-22]
METTL14 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22666-22] to METTL14 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: 293T, HeLa, NIH/3T3, C6, HeLa and HeLa transfected with siRNA specifically targeting MettL14, whole lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22666-22 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300104の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.
ターゲット情報
-
機能
N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. -
配列類似性
Belongs to the MT-A70-like family. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 57721 Human
- Entrez Gene: 210529 Mouse
- Entrez Gene: 295428 Rat
- Omim: 616504 Human
- SwissProt: Q9HCE5 Human
- SwissProt: Q3UIK4 Mouse
- Unigene: 596872 Human
- Unigene: 657806 Human
see all -
別名
- KIAA1627 antibody
- MET14_HUMAN antibody
- Methyltransferase like 14 antibody
see all
画像
-
Western blot - Anti-METTL14 antibody [EPR22666-22] (AB300104)All lanes : Anti-METTL14 antibody [EPR22666-22] (ab300104) at 1/5000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24316715).
There is an unknown band at ~50kDa. -
![Western blot - Anti-METTL14 antibody [EPR22666-22] (ab300104)](https://www.abcam.co.jp/ps/products/300/ab300104/Images/AB300104-485992-WB1.jpg)
Western blot - Anti-METTL14 antibody [EPR22666-22] (ab300104)All lanes : Anti-METTL14 antibody [EPR22666-22] (ab300104) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting MettL14, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 24316715).
There is an unknown band at ~50kDa.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range. -
![Anti-METTL14 antibody [EPR22666-22] (ab300104)](https://www.abcam.co.jp/ps/products/300/ab300104/Images/AB300104-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-METTL14 antibody [EPR22666-22] (ab300104)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (12)
ab300104 は 12 報の論文で使用されています。
- Wang W et al. Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis. Biomol Biomed 24:505-519 (2024). PubMed: 37902450
- Zhao M et al. N6-methyladenosine modification of TSC1 mRNA contributes to macrophage polarization regulated by Coptisine in DSS-induced ulcerative colitis. Phytomedicine 122:155153 (2024). WB ; Mouse . PubMed: 38014839
- Chen F et al. METTL14 depletion ameliorates ferroptosis in severe acute pancreatitis by increasing the N6-methyladenosine modification of ACSL4 and STA1. Int Immunopharmacol 128:111495 (2024). WB ; Mouse . PubMed: 38237228
- Jin J et al. Exosomes as nanostructures deliver miR-204 in alleviation of mitochondrial dysfunction in diabetic nephropathy through suppressing methyltransferase-like 7A-mediated CIDEC N6-methyladenosine methylation. Aging (Albany NY) 16:3302-3331 (2024). WB ; Human . PubMed: 38334961
- Li L et al. METTL14 decreases FTH1 mRNA stability via m6A methylation to promote sorafenib-induced ferroptosis of cervical cancer. Cancer Biol Ther 25:2349429 (2024). WB ; Human . PubMed: 38738555