Anti-METTL13 抗体 (ab186002)
Key features and details
- Rabbit polyclonal to METTL13
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-METTL13 antibody
METTL13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to METTL13 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human METTL13. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_057019.3
Database link: Q8N6R0 -
ポジティブ･コントロール
- HeLa, 293T or Jurkat lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186002の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 79 kDa. |
ターゲット情報
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配列類似性
Belongs to the methyltransferase superfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 456446 Chimpanzee
- Entrez Gene: 51603 Human
- Entrez Gene: 100432314 Orangutan
- SwissProt: Q8N6R0 Human
- Unigene: 494705 Human
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別名
- 5630401D24Rik antibody
- CGI-01 antibody
- MET13_HUMAN antibody
see all
画像
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Immunoprecipitation - Anti-METTL13 antibody (ab186002)
Detection of Human METTL13 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab186002 used for IP at 1 µg/ml. For blotting immunoprecipitated METTL13, ab186002 was used at 1 µg/ml.
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Western blot - Anti-METTL13 antibody (ab186002)All lanes : Anti-METTL13 antibody (ab186002) at 0.4 mg/ml
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 79 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab186002 は 3 報の論文で使用されています。
- Wu Q et al. METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit. J Cell Commun Signal 17:121-135 (2023). PubMed: 35925508
- Liu Z et al. METTL13 inhibits progression of clear cell renal cell carcinoma with repression on PI3K/AKT/mTOR/HIF-1a pathway and c-Myc expression. J Transl Med 19:209 (2021). PubMed: 33985542
- Cen S et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273