Anti-METTL1 抗体 (ab157097)
Key features and details
- Rabbit polyclonal to METTL1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-METTL1 antibody
METTL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to METTL1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Common marmoset, Orangutan, Platypus
-
免疫原
Synthetic peptide corresponding to a region within amino acids 175-225 of Human METTL1 (NP_005362.3).
-
ポジティブ･コントロール
- 293T, HeLa, Jurkat, and TCMK-1 whole cell lysates
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157097の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 31 kDa. |
ターゲット情報
-
機能
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. -
組織特異性
Ubiquitous. -
パスウェイ
tRNA modification; N(7)-methylguanine-tRNA biosynthesis. -
配列類似性
Belongs to the methyltransferase superfamily. TrmB family. -
翻訳後修飾
Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 4234 Human
- Entrez Gene: 17299 Mouse
- Entrez Gene: 715796 Rhesus monkey
- GenBank: BC000550 Human
- Omim: 604466 Human
- SwissProt: Q9UBP6 Human
- SwissProt: Q9Z120 Mouse
- Unigene: 42957 Human
see all -
別名
- C12orf1 antibody
- D1075 like gene product antibody
- FLJ95748 antibody
see all
画像
-
Western blot - Anti-METTL1 antibody (ab157097)All lanes : Anti-METTL1 antibody (ab157097) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 3 minutes
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab157097 は 2 報の論文で使用されています。
- Xie H et al. METTL1 drives tumor progression of bladder cancer via degrading ATF3 mRNA in an m7G-modified miR-760-dependent manner. Cell Death Discov 8:458 (2022). PubMed: 36396627
- Pandolfini L et al. METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation. Mol Cell 74:1278-1290.e9 (2019). PubMed: 31031083