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Anti-MetRS/MARS 抗体 [MARSD10B4] (ab50793)

Submit a review Submit a question References (9)
Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
  • Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
  • Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
  • Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
  • Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
  • Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)

Key features and details

  • Mouse monoclonal [MARSD10B4] to MetRS/MARS
  • Suitable for: WB, Flow Cyt, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MetRS/MARS antibody [MARSD10B4]
    MetRS/MARS 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MARSD10B4] to MetRS/MARS

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, Flow Cyt, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment corresponding to Human MetRS/MARS aa 50-200 (C terminal).

  • ポジティブ･コントロール

    • WB: NIH3T3 whole cell, HeLa whole cell and F2408 whole cell lysates. IP: RIPA lysate of HeLa cells. ICC: HeLa cell. Flow Cyt: Hela cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 1% BSA
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Ab50793 was filtered through an 0.22 µm membrane.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MARSD10B4

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab50793の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/100. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Flow Cyt
1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/10.
IP
Use at 5 µg/mg of lysate.
特記事項
WB
1/100. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Flow Cyt
1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/10.
IP
Use at 5 µg/mg of lysate.

ターゲット情報

画像

  • Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 101 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Immunoprecipitation from 200ug RIPA lysate of HeLa cells using 5ug of ab50793. Visualized by Western Blot using ab50793 at 1/100 dilution, anti-mouse IgG secondary 1/1000 and ECL detection.

    Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 5 µg + RIPA lysate of HeLa cells at 200 µg

    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 101 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody

    Predicted band size: 101 kDa
    Observed band size: 98 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)

    Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.

  • Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
    Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)

    Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cells by Flow Cytometry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/400.

参考文献 (9)

ab50793 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab50793 は 9 報の論文で使用されています。

  • Zhao R  et al. Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease. Cell Rep Med 4:100953 (2023). PubMed: 36809766
  • Yoon I  et al. EPRS1 Controls the TGF-β Signaling Pathway via Interaction with TβRI in Hepatic Stellate Cell. Mol Cell Biol 43:223-240 (2023). PubMed: 37154023
  • Theodoridis PR  et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33568529
  • Silva RC  et al. A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2a. MethodsX 5:75-82 (2018). PubMed: 30619721
  • Wang D  et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
  • Kwon NH  et al. Stabilization of Cyclin-Dependent Kinase 4 by Methionyl-tRNA Synthetase in p16INK4a-Negative Cancer. ACS Pharmacol Transl Sci 1:21-31 (2018). PubMed: 32219202
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . PubMed: 26544075
  • Gulati P  et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB, IP ; Mouse . PubMed: 23359686
  • Yannay-Cohen N  et al. LysRS serves as a key signaling molecule in the immune response by regulating gene expression. Mol Cell 34:603-11 (2009). WB ; Rat . PubMed: 19524539

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