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リコンビナントRabMAb

Anti-Methionyl Aminopeptidase 1/MAP 1 抗体 [EPR15452(B)] (ab185540)

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Flow Cytometry (Intracellular) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
  • Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)]
    Methionyl Aminopeptidase 1/MAP 1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15452(B)] to Methionyl Aminopeptidase 1/MAP 1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • A375 and HUVEC lysate. Human cervix carcinoma and Rat skeletal muscle tissue. A549 and A375 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185540の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

ターゲット情報

画像

  • Flow Cytometry (Intracellular) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Flow Cytometry (Intracellular) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    All lanes : Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)
    Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (ab185540)

参考文献 (0)

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ab185540 は論文での使用が確認できていません。

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