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リコンビナントRabMAb

Anti-MERTK 抗体 [EPR26359-12] (BSA and Azide free) (ab300137)

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Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
  • Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26359-12] to MERTK - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-MERTK antibody [EPR26359-12] (BSA and Azide free)
    MERTK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26359-12] to MERTK - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole tissue lysates: mouse spleen, HepG2 (human hepatocellular carcinoma epithelial cell), J774A.1 (mouse reticulum cell sarcoma monocyte macrophage). IHC-P: Mouse kidney and Mouse spleen tissues. ICC/IF: J774A.1 cells. Flow Cyt.: HepG2, J774A.1 and NR8383 cells.

  • 特記事項

    Ab300137 us a carrier free version of ab300136. 

    WB and ICC do not reactive with Rat species. IHC application does not reactive with Human and  Rat species. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300137の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.

For Western Blot, it is recommended to use ab52968 for detecting human samples and ab184086 for detecting mouse samples.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.

For Western Blot, it is recommended to use ab52968 for detecting human samples and ab184086 for detecting mouse samples.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    In case of filovirus infection, seems to function as a cell entry factor.

  • 組織特異性

    Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.

  • 関連疾患

    Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • c MER antibody
    • c mer proto oncogene tyrosine kinase antibody
    • c-mer antibody
    • cMER antibody
    • cmer protooncogene tyrosine kinase antibody
    • Eyk antibody
    • MER antibody
    • MER receptor tyrosine kinase antibody
    • MERK antibody
    • MERPEN antibody
    • Mertk antibody
    • MERTK c-mer proto-oncogene tyrosine kinase antibody
    • MERTK_HUMAN antibody
    • MGC133349 antibody
    • nmf12 antibody
    • Nyk antibody
    • Proto oncogene tyrosine protein kinase MER antibody
    • Proto oncogene tyrosine protein kinase MER precursor antibody
    • Proto-oncogene c-Mer antibody
    • Receptor tyrosine kinase MerTK antibody
    • RP38 antibody
    • STK kinase antibody
    • Tyrosine-protein kinase Mer antibody
    see all

画像

  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
    Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
    All lanes : Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution (ab52968 1/1000
    ab184086 1/1000
    ab181602 1/1000000)

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110-140,180-210 kDa why is the actual band size different from the predicted?


    Exposure time: 140 seconds


    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    For Western Blot, it is recommended to use ab52968 for detecting human samples and ab184086 for detecting mouse samples.

  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
    Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
    All lanes : Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110-140,180-210 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: ECL western blotting substrate 180 seconds, higher sensitivity ECL western substrate 80 seconds.

    The left image uses the ECL western blotting substrate and the right image uses the higher sensitivity ECL western substrate.

    We recommend using a higher sensitive ECL substrate to increase the band intensity.

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    All lanes : Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate
    Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110-140,180-210 kDa why is the actual band size different from the predicted?


    Exposure time: 92 seconds


    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    Negative control: WEHI-231 (PMID: 12884290)
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16652142)
    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    All lanes : Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : HL-60 (human acute promyelocytic leukemia promyeloblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110-140,180-210 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    Exposure time: 3 minutes. 

    Negative control: HL-60 (PMID:23474756)
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16652142)
    This blot was developed using a higher sensitivity ECL substrate.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on endothelial cells of mouse kidney (PMID: 29350876) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on mouse spleen (PMID: 25479139) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). 

  • Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)
    Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (ab300137)

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte macrophage) cells labeling MERTK with ab300136 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in J774A.1 cell line. Negative control: WEHI-231(PMID: 16652142; PMID:12884290).

    Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution.

  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of HL-60 (human acute promyelocytic leukemia promyeloblast, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HL-60(PMID:23474756). 

  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of WEHI-231 (mouse B cell lymphoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells labelling MERTK with ab300136 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control: WEHI-231(PMID:16652142; PMID:12884290)

  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

    This data was developed using ab300136, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of NR8383 (rat lung macrophage alveolar) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)
    Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300137 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300137 は論文での使用が確認できていません。

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