Anti-Meprin beta 抗体 (ab42743)
Key features and details
- Rabbit polyclonal to Meprin beta
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Meprin beta antibody
Meprin beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Meprin beta -
由来種
Rabbit -
特異性
Meprin beta antibody Math Domain recognizes meprin beta but not alpha subunits. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Peptide-affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42743の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 84 kDa.
1/1000 for using colorimetric substrates such as BCIP/NBT. WB: 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Dilution optimised using Chromogenic detection. |
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 84 kDa. 1/1000 for using colorimetric substrates such as BCIP/NBT. WB: 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
Membrane metallopeptidase that sheds many membrane-bound proteins. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. -
組織特異性
The major site of expression is the brush border membrane of small intestinal and kidney epithelial cells. -
配列類似性
Belongs to the peptidase M12A family.
Contains 1 EGF-like domain.
Contains 1 MAM domain.
Contains 1 MATH domain. -
翻訳後修飾
N-glycosylated; contains high mannose and/or complex biantennary structures.
O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human.
Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues. -
細胞内局在
Cell membrane. Secreted. Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17. - Information by UniProt
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参照データベース
- Entrez Gene: 4225 Human
- Omim: 600389 Human
- SwissProt: Q16820 Human
- Unigene: 194777 Human
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別名
- Endopeptidase 2 antibody
- Endopeptidase-2 antibody
- kinase splitting membranal proteinase antibody
see all
画像
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Western blot - Anti-Meprin beta antibody (ab42743)All lanes : Anti-Meprin beta antibody (ab42743) at 1/1000 dilution
Lane 1 : recombinant Human Meprin-alpha at 0.05 µg
Lane 2 : negative control
Lane 3 : recombinant Human Meprin-beta at 0.05 µg
Predicted band size: 84 kDa
Observed band size: 84 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab42743 は 2 報の論文で使用されています。
- Gao R et al. Meprin-a (Mep1A) enhances TNF-a secretion by mast cells and aggravates abdominal aortic aneurysms. Br J Pharmacol 177:2872-2885 (2020). PubMed: 32072633
- Livingston MJ et al. Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction. Autophagy 12:976-98 (2016). PubMed: 27123926