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Anti-MEPCE 抗体 - C-terminal (ab185991)

Submit a review Submit a question References (1)
Immunoprecipitation - Anti-MEPCE antibody - C-terminal (ab185991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEPCE antibody - C-terminal (ab185991)

Key features and details

  • Rabbit polyclonal to MEPCE - C-terminal
  • Suitable for: IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-MEPCE antibody - C-terminal

  • 製品の詳細

    Rabbit polyclonal to MEPCE - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan

  • 免疫原

    Synthetic peptide within Human MEPCE aa 600 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_062552.2.
    Database link: Q7L2J0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • IP: 293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    ab185991 was affinity purified using an epitope specific to MEPCE immobilized on solid support.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185991の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 2-10 µg/mg of lysate.
特記事項
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 関連性

    MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.

  • 参照データベース

    • 別名

      • 7SK snRNA methylphosphate capping enzyme antibody
      • BCDIN3 antibody
      • Bicoid interacting protein 3 homolog antibody
      • Bin3 bicoid interacting 3 antibody
      • Bin3 bicoid interacting 3 homolog (Drosophila) antibody
      • Bin3 bicoid interacting 3 homolog antibody
      • Bin3 homolog antibody
      • FLJ20257 antibody
      • MePCE antibody
      • Methylphosphate capping enzyme antibody
      see all

    画像

    • Immunoprecipitation - Anti-MEPCE antibody - C-terminal (ab185991)
      Immunoprecipitation - Anti-MEPCE antibody - C-terminal (ab185991)

      Detection of MEPCE in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185991 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-MEPCE antibody was used at 1µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEPCE antibody - C-terminal (ab185991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEPCE antibody - C-terminal (ab185991)

      Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling MEPCE with ab185991 at a dilution of 1/1000.

    参考文献 (1)

    ab185991 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185991 は 1 報の論文で使用されています。

    • Egloff S  et al. The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression. EMBO J 36:934-948 (2017). PubMed: 28254838

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