Anti-Melanopsin 抗体 (ab19306)
Key features and details
- Rabbit polyclonal to Melanopsin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Melanopsin antibody
Melanopsin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Melanopsin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Melanopsin aa 1-100.
Database link: Q8R456 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19306の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. -
組織特異性
Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. -
細胞内局在
Cell membrane. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells. - Information by UniProt
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参照データベース
- Entrez Gene: 94233 Human
- Entrez Gene: 30044 Mouse
- Entrez Gene: 192223 Rat
- Omim: 606665 Human
- SwissProt: Q14D01 Human
- SwissProt: Q9UHM6 Human
- SwissProt: Q9QXZ9 Mouse
- SwissProt: Q8R456 Rat
see all -
別名
- Melanopsin antibody
- MGC142118 antibody
- MOP antibody
see all
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab19306 は 6 報の論文で使用されています。
- Xu X et al. Effects of artificial light with different spectral composition on eye axial growth in juvenile guinea pigs. Eur J Histochem 67:N/A (2023). PubMed: 36786079
- Benedetto MM et al. Non-Visual Photopigments Effects of Constant Light-Emitting Diode Light Exposure on the Inner Retina of Wistar Rats. Front Neurol 8:417 (2017). PubMed: 28871236
- Lu Q & Pan ZH Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines. Methods Mol Biol 1642:181-194 (2017). PubMed: 28815501
- Liu M et al. Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Mol Med Rep 13:321-6 (2016). PubMed: 26572076
- Jain V et al. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS One 11:e0157226 (2016). PubMed: 27295136
- Boudard DL et al. Loss of photic entrainment at low illuminances in rats with acute photoreceptor degeneration. Eur J Neurosci 30:1527-36 (2009). PubMed: 19821841