Anti-Melanophilin 抗体 (ab2716)
Key features and details
- Goat polyclonal to Melanophilin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Melanophilin antibody -
製品の詳細
Goat polyclonal to Melanophilin -
由来種
Goat -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Mouse Melanophilin aa 550 to the C-terminus (C terminal).
Database link: Q91V27 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2716の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
1 hour primary incubation is recommended for this product. |
|
| ICC/IF |
Use a concentration of 0.5 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa. 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 0.5 µg/ml. |
ターゲット情報
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機能
Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. -
関連疾患
Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. -
配列類似性
Contains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 171531 Mouse
- Entrez Gene: 316620 Rat
- SwissProt: Q91V27 Mouse
- Unigene: 105208 Mouse
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別名
- 2210418F23Rik antibody
- 5031433I09Rik antibody
- AW228792 antibody
see all
画像
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Western blot - Anti-Melanophilin antibody (ab2716)All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml
Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice
Predicted band size: 65 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?ab2716 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface.
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Immunocytochemistry/ Immunofluorescence - Anti-Melanophilin antibody (ab2716)Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab2716 は 1 報の論文で使用されています。
- Kudo M et al. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS One 12:e0171513 (2017). PubMed: 28182699
