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リコンビナントRabMAb

Anti-Melanoma Inhibitory Activity 抗体 [EPR15597] (ab186731)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)
  • Western blot - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)
  • Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15597] to Melanoma Inhibitory Activity
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Melanoma Inhibitory Activity antibody [EPR15597]
    Melanoma Inhibitory Activity 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15597] to Melanoma Inhibitory Activity

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WM-266-4 and A375 cell lysates; Human melanoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186731の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

  • 組織特異性

    All malignant melanoma cell lines tested and infrequently in glioma cell lines.

  • 配列類似性

    Belongs to the MIA/OTOR family.
    Contains 1 SH3 domain.

  • 翻訳後修飾

    May possess two intramolecular disulfide bonds.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cartilage derived retinoic acid sensitive protein antibody
      • CD RAP antibody
      • CDRAP antibody
      • Melanoma derived growth regulatory protein precursor antibody
      • Melanoma inhibitory activity protein antibody
      • Melanoma-derived growth regulatory protein antibody
      • Mia antibody
      • MIA_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)

      Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling Melanoma Inhibitory Activity with ab186731 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)
      Western blot - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)
      All lanes : Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731) at 1/1000 dilution

      Lane 1 : WM-266-4 cell lysate
      Lane 2 : A375 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 15 kDa

    • Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)
      Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)

    参考文献 (1)

    ab186731 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186731 は 1 報の論文で使用されています。

    • Cvetanova B  et al. Sesquiterpene Lactone Deoxyelephantopin Isolated from Elephantopus scaber and Its Derivative DETD-35 Suppress BRAFV600E Mutant Melanoma Lung Metastasis in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33810045

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