AB137062

Anti-Melanoma gp100 抗体 [EPR4864]

Name: Anti-Melanoma gp100 抗体 [EPR4864] (ab137062) | Abcam
Brand: Abcam Limited
SKU: AB137062
Price: 53000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Melanoma gp100 antibody [EPR4864]

RabMAb
Recombinant
詳細を見る

(1 Review)

(11 Publications)

Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit monoclonal antibody detecting Melanoma gp100 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

別名を表示する

D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical staining of paraffin embedded human melanoma with purified ab137062 at a working dilution of 1 in 400. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical staining of paraffin embedded human melanoma with unpurified ab137062 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Unknown

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

All lanes:

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/1000 dilution

All lanes:

Human melanoma tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 70 kDa

false

Lab

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/10000 dilution

All lanes:

Human melanoma tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 100 kDa,26 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal stomach tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurified ab137062 showing negative staining in placenta tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurifed ab137062 showing negative staining in normal skin tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4864

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "For unpurified, use 1/50 - 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified, use 1/1000 - 1/2000." } } }

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製品の詳細

What is this antibody validated in?
Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Melanoma gp100?
Anti-Melanoma gp100 [EPR4864] (ab137062) specifically detects a band for Melanoma gp100 (UniProt: P40967) at a molecular weight of 70kDa.

Trusted by the scientific community
Anti-Melanoma gp100 [EPR4864] (ab137062) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4864] also available for your convenience: ab137062, Carrier free - ab190913, HRP - ab206094, APC - ab319326, PE - ab319463, Alexa Fluor® 488 - ab319583, Alexa Fluor® 647 - ab319689, Alexa Fluor® 594 - ab319819, Alexa Fluor® 555 - ab319960, Alexa Fluor® 750 - ab321106

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.

See full target information PMEL

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

SLAS technology 29:100106 PubMed37657710

2023

A semi-automated pipeline for quantifying drusen-like deposits in human induced pluripotent stem cell-derived retinal pigment epithelium cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna Hall,Maciej Daniszewski,Shane Cheung,Kalyan Shobhana,Himeesh Kumar,Helena H Liang,Henry Beetham,Ellie Cho,Carla Abbott,Alex W Hewitt,Kaylene J Simpson,Robyn H Guymer,Daniel Paull,Alice Pébay,Grace E Lidgerwood

Nature communications 13:4233 PubMed35882847

2022

Transcriptomic and proteomic retinal pigment epithelium signatures of age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Senabouth,Maciej Daniszewski,Grace E Lidgerwood,Helena H Liang,Damián Hernández,Mehdi Mirzaei,Stacey N Keenan,Ran Zhang,Xikun Han,Drew Neavin,Louise Rooney,Maria Isabel G Lopez Sanchez,Lerna Gulluyan,Joao A Paulo,Linda Clarke,Lisa S Kearns,Vikkitharan Gnanasambandapillai,Chia-Ling Chan,Uyen Nguyen,Angela M Steinmann,Rachael A McCloy,Nona Farbehi,Vivek K Gupta,David A Mackey,Guy Bylsma,Nitin Verma,Stuart MacGregor,Matthew J Watt,Robyn H Guymer,Joseph E Powell,Alex W Hewitt,Alice Pébay

Regenerative therapy 19:24-34 PubMed35059477

2022

Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture.

Applications

Unspecified application

Species

Unspecified reactive species

Suguru Yamasaki,Atsushi Kuwahara,Akiyoshi Kishino,Toru Kimura,Masayo Takahashi,Michiko Mandai

Scientific reports 11:7730 PubMed33833328

2021

Identification of critical amino acid residues in the regulatory N-terminal domain of PMEL.

Applications

Unspecified application

Species

Unspecified reactive species

Susan M Mitchell,Morven Graham,Xinran Liu,Ralf M Leonhardt

Methods in molecular biology (Clifton, N.J.) 2265:213-222 PubMed33704717

2021

Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron B Beasley,Emmanuel Acheampong,Weitao Lin,Elin S Gray

Scientific reports 9:6101 PubMed30988362

2019

Repeat domain-associated O-glycans govern PMEL fibrillar sheet architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Morven Graham,Athanasia C Tzika,Susan M Mitchell,Xinran Liu,Ralf M Leonhardt

Cell cycle (Georgetown, Tex.) 17:844-857 PubMed29623762

2018

Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Juan Yi,Meng-Yun Su,Ying Shi,Shan Jiang,Shi-Zheng Xu,Tie-Chi Lei

Investigative ophthalmology & visual science 59:1374-1383 PubMed29625461

2018

Allogeneic iPSC-Derived RPE Cell Graft Failure Following Transplantation Into the Subretinal Space in Nonhuman Primates.

Applications

Unspecified application

Species

Macaque monkey

Trevor J McGill,Jonathan Stoddard,Lauren M Renner,Ilhem Messaoudi,Kapil Bharti,Shoukhrat Mitalipov,Andreas Lauer,David J Wilson,Martha Neuringer

The Journal of investigative dermatology 138:171-178 PubMed28842328

2017

Melanocytes Sense Blue Light and Regulate Pigmentation through Opsin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Regazzetti,Laura Sormani,Delphine Debayle,Françoise Bernerd,Meri K Tulic,Gian Marco De Donatis,Bérengère Chignon-Sicard,Stéphane Rocchi,Thierry Passeron

Translational vision science & technology 6:17 PubMed28626601

2017

Long-Term Efficacy of GMP Grade Xeno-Free hESC-Derived RPE Cells Following Transplantation.

Applications

IHC-P

Species

Rat

Trevor J McGill,Osnat Bohana-Kashtan,Jonathan W Stoddard,Michael D Andrews,Neelay Pandit,Lior R Rosenberg-Belmaker,Ofer Wiser,Limor Matzrafi,Eyal Banin,Benjamin Reubinoff,Nir Netzer,Charles Irving

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Melanoma gp100 抗体 [EPR4864]

Anti-Melanoma gp100 antibody [EPR4864]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

製品プロトコール

ターゲットの情報

文献 (11)

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