Anti-Melanoma Associated Antigen 100+ / 7 kDa 抗体 [NKI/beteb] (ab34165)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] (ab34165)](https://www.abcam.co.jp/ps/products/34/ab34165/Images/ab34165-47536-anti-melanoma-associated-antigen-100-7-kda-antibody-nki-beteb-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] (ab34165)")
Key features and details
- Mouse monoclonal [NKI/beteb] to Melanoma Associated Antigen 100+ / 7 kDa
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] -
製品の詳細
Mouse monoclonal [NKI/beteb] to Melanoma Associated Antigen 100+ / 7 kDa -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Melanoma Associated Antigen 100+/ 7 kDa. Membranes from a human melanoma metastasis
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
NKI/beteb -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
Also see PubMed: 21247888
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| IHC-P |
1/5 - 1/20.
Formalin fixation and embedding in paraffin may affect the reactivity of the antibody. |
| 特記事項 |
|---|
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Flow Cyt
Use 1µg for 106 cells. Also see PubMed: 21247888
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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IHC-P
1/5 - 1/20. Formalin fixation and embedding in paraffin may affect the reactivity of the antibody. |
ターゲット情報
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関連性
A (pre)melanosomal 100 + 7 kd antigen (glycoprotein) present in melanomas, clear cells sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. Except for one case of non Hodgkin's lymphoma in which macrophages were positive, the antigen has not been detected with other tumors or tissues observed. -
細胞内局在
The innerside of membranes of cytoplasmic vesicles in melanoma cells. -
参照データベース
- SwissProt: Human
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別名
- MAA antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] (ab34165)IHC image of ab34165 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34165 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] (ab34165)](https://www.abcam.co.jp/ps/products/34/ab34165/Images/ab34165-47535-anti-melanoma-associated-antigen-100-7-kda-antibody-nki-beteb-flow-cytometry.jpg)
Flow Cytometry - Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb] (ab34165)Overlay histogram showing Malme-3 cells stained with ab34165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab34165, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab34165 は 9 報の論文で使用されています。
- Phua R et al. In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic. STAR Protoc 4:102334 (2023). PubMed: 37243599
- Altonsy MO et al. Beta3-Tubulin is Critical for Microtubule Dynamics, Cell Cycle Regulation, and Spontaneous Release of Microvesicles in Human Malignant Melanoma Cells (A375). Int J Mol Sci 21:N/A (2020). PubMed: 32121295
- Sridharan A et al. Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis. Methods Mol Biol 2109:55-65 (2020). PubMed: 31161578
- Bissig C et al. The PIKfyve complex regulates the early melanosome homeostasis required for physiological amyloid formation. J Cell Sci 132:N/A (2019). PubMed: 30709920
- Lo Cicero A et al. Pathological modelling of pigmentation disorders associated with Hutchinson-Gilford Progeria Syndrome (HGPS) revealed an impaired melanogenesis pathway in iPS-derived melanocytes. Sci Rep 8:9112 (2018). PubMed: 29904107
- Bergam P et al. ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation. J Mol Biol N/A:N/A (2018). PubMed: 29940187
- Leonhardt RM et al. Proprotein convertases process Pmel17 during secretion. J Biol Chem 286:9321-37 (2011). Flow Cyt ; Human . PubMed: 21247888
- Leonhardt RM et al. Endoplasmic reticulum export, subcellular distribution, and fibril formation by Pmel17 require an intact N-terminal domain junction. J Biol Chem 285:16166-83 (2010). ICC/IF ; Human . PubMed: 20231267
- Vennegoor C et al. A monoclonal antibody specific for cells of the melanocyte lineage. Am J Pathol 130:179-92 (1988). PubMed: 3276209