Anti-Melanoma 抗体 [PNL2] (ab12502)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)](https://www.abcam.co.jp/ps/products/12/ab12502/Images/ab12502-20060-anti-melanoma-antibody-pnl2-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)")
Key features and details
- Mouse monoclonal [PNL2] to Melanoma
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Melanoma antibody [PNL2]
Melanoma 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PNL2] to Melanoma -
由来種
Mouse -
特異性
This antibody labels melanocytes in the cytoplasm and plasma membrane and is useful tool for the identification of melanomas and clear cell sarcomas. It seems to be a melanocyte antibody of autoimmune origin as it does not react with the non-melanocytic peptide antigen used for immunization. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ･コントロール
- Melanoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
PNL2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12502の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites. -
細胞内局在
Membrane; Single-pass membrane protein -
参照データベース
- Omim: 605513 Human
- SwissProt: Q16655 Human
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別名
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)IHC image of ab12502 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12502, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)](https://www.abcam.co.jp/ps/products/12/ab12502/Images/ab12502-295035-anti-melanoma-antibody-pnl2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (ab12502)Formalin-fixed, paraffin-embedded human melanoma tissue stained for Melanoma using ab12502 at 1/50 dilution in immunohistochemical analysis.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab12502 は 4 報の論文で使用されています。
- Moroki T et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol 34:161-180 (2021). PubMed: 33976473
- Needle DB et al. Biphasic malignant melanoma adenocarcinoma in the digit of a dog. J Vet Diagn Invest 30:315-318 (2018). PubMed: 29284381
- Furukawa S et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rat . PubMed: 28190929
- Rochaix P et al. PNL2, a new monoclonal antibody directed against a fixative-resistant melanocyte antigen. Mod Pathol 16:481-90 (2003). PubMed: 12748255