Anti-MelanA 抗体 [M2-9E3] (ab187369)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)](https://www.abcam.co.jp/ps/products/187/ab187369/Images/ab187369-219586-anti-melana-antibody-m29e3-immunohistochemistry-melanoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)")
Key features and details
- Mouse monoclonal [M2-9E3] to MelanA
- Suitable for: IP, IHC-Fr, ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-MelanA antibody [M2-9E3]
MelanA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M2-9E3] to MelanA -
由来種
Mouse -
アプリケーション
適用あり: IP, IHC-Fr, ICC/IF, Flow Cyt, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human MelanA aa 1 to the C-terminus.
Database link: Q16655 -
ポジティブ･コントロール
- IHC-P: Human melanoma tissue. WB: COLO38 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
ab187369 was purified from Bioreactor Concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
M2-9E3 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 13 kDa.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 13 kDa. |
ターゲット情報
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機能
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. -
組織特異性
Expression is restricted to melanoma and melanocyte cell lines and retina. -
翻訳後修飾
Acylated. -
細胞内局在
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation. - Information by UniProt
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参照データベース
- Entrez Gene: 2315 Human
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
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別名
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling MelanA with ab187369 at 1 µg/ml.
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![Western blot - Anti-MelanA antibody [M2-9E3] (ab187369)](https://www.abcam.co.jp/ps/products/187/ab187369/Images/ab187369-453194-anti-melana-antibody-m29e3-western-blot-colo38-human.jpg)
Western blot - Anti-MelanA antibody [M2-9E3] (ab187369)Anti-MelanA antibody [M2-9E3] (ab187369) at 1 µg/ml + Human COLO38 cell lysate
Predicted band size: 13 kDaWestern blot analysis of COLO38 cell lysate labeling MelanA with ab187369 at 1 ug/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)](https://www.abcam.co.jp/ps/products/187/ab187369/Images/ab187369-219583-anti-melana-antibody-m29e3-immunohistochemistry-melanoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling MelanA with ab187369 at 1 µg/ml.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab187369 は 7 報の論文で使用されています。
- Li H et al. Renal epithelioid angiomyolipoma: A case report. Oncol Lett 26:409 (2023). PubMed: 37600337
- Kang P et al. Oxeiptosis: a novel pathway of melanocytes death in response to oxidative stress in vitiligo. Cell Death Discov 8:70 (2022). PubMed: 35177586
- Yamagiwa Y et al. Orbital malignant schwannoma and embryonal rhabdomyosarcoma in rats caused by leakage of locally injected nickel subsulfide to the orbit. J Toxicol Pathol 34:151-156 (2021). PubMed: 33976471
- Chen X et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
- Guo W et al. Up-regulated deubiquitinase USP4 plays an oncogenic role in melanoma. J Cell Mol Med 22:2944-2954 (2018). PubMed: 29542252
- Yang CF et al. Loss of GPNMB Causes Autosomal-Recessive Amyloidosis Cutis Dyschromica in Humans. Am J Hum Genet 102:219-232 (2018). PubMed: 29336782
- Guo B et al. Malignant renal epithelioid angiomyolipoma: A case report and review of the literature. Oncol Lett 11:95-98 (2016). PubMed: 26870174
