Anti-MEKK3 抗体 [ep600y] (ab40756)

Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

Exposure Time:

Lane 1: 92 seconds

Lane 2: 180 seconds

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In lane 2, the lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

71 kDa MEKK3 was immunoprecipitated from 0.35mg of bEnd.3 (mouse brain endothelial cell) whole cell lysate with ab40756 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40756 at 1/200 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 (Input): bEnd.3 (mouse brain endothelial cell) whole cell lysate 10 μg.

Lane 2 (+): bEnd.3 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40756 in bEnd.3 whole cell lysate

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 180 seconds.

Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1:100

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEKK3 with purified ab40756 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling MEKK3 with purified ab40756 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).