AB40756

Anti-MEKK3 抗体 [ep600y]

Anti-MEKK3 antibody [ep600y]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(10 Publications)

Rabbit Recombinant Monoclonal MEKK3 antibody. Suitable for IHC-P, IP, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 10 publications.

別名を表示する

MAPKKK3, MEKK3, MAP3K3, Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3

5 Images

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] (AB40756)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] (AB40756)

Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling MEKK3 with purified ab40756 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody [ep600y] (AB40756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody [ep600y] (AB40756)

Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1 : 100

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] (AB40756)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEKK3 with purified ab40756 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-MEKK3 antibody [ep600y] (AB40756)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MEKK3 antibody [ep600y] (AB40756)

Immunofluorescent staining of HeLa cells using ab40756 diluted 1 : 100

Lab

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] (AB40756)

71 kDa MEKK3 was immunoprecipitated from 0.35mg of bEnd.3 (mouse brain endothelial cell) whole cell lysate with ab40756 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40756 at 1/200 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 (Input) : bEnd.3 (mouse brain endothelial cell) whole cell lysate 10 μg. Lane 2 (+) : bEnd.3 whole cell lysate. Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab40756 in bEnd.3 whole cell lysate Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] (ab40756) at 1/200 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

ep600y

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

IHC-P, Flow Cyt (Intra), ICC/IF, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20 - 1/30", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/30", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MEKK3 also known as MAP3K3 is a serine/threonine kinase that plays an important role in the MAPK signaling cascade. This protein has a molecular mass of approximately 67 kDa. MEKK3 is widely expressed with notable presence in tissues like the heart placenta and skeletal muscles. It is important for transmitting signals from the cell surface to various intracellular targets impacting numerous cellular processes.

Biological function summary

MEKK3 participates in cell growth differentiation and apoptosis regulation. It often functions within a multi-protein complex including components like MEK5 and ERK5. MEKK3 activates downstream kinases influencing gene expression and cellular responses to external signals. Its involvement is essential for controlling reactions to stress and inflammatory stimuli.

Pathways

MEKK3 integrates into the MAPK and NF-κB pathways both critical for mediating signaling responses. In the MAPK pathway MEKK3 regulates kinases like MEK5 and ERK5. Additionally it connects with proteins such as IKK in the NF-κB pathway influencing immunity and cell survival. These interactions position MEKK3 centrally in cellular defense and adaptability mechanisms.

MEKK3 associates with pathological conditions like cardiovascular diseases and cancer. Its role in these disorders links closely with dysregulation in the MAPK pathway where MEK5 and ERK5 also play significant roles. Furthermore aberrations in the NF-κB pathway involving IKK can contribute to its involvement in these diseases highlighting MEKK3's impact on disease progression and potential as a therapeutic target.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

See full target information MAP3K3

style

left-column

文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:1146 PubMed34887384

2021

ETV5-mediated upregulation of lncRNA CTBP1-DT as a ceRNA facilitates HGSOC progression by regulating miR-188-5p/MAP3K3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Liu,Ruiting Fu,Kai Chen,Lu Zhang,Shasha Wang,Weihua Liang,Hong Zou,Lin Tao,Wei Jia

Frontiers in cell and developmental biology 9:570451 PubMed33644040

2021

MiR-132-3p Modulates MEKK3-Dependent NF-κB and p38/JNK Signaling Pathways to Alleviate Spinal Cord Ischemia-Reperfusion Injury by Hindering M1 Polarization of Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Fang,Hua-Feng Li,Qin Pan,Hon-Ling Jin,Miao Yang,Ru-Rong Wang,Quan-Yun Wang,Jian-Ping Zhang

Journal of experimental & clinical cancer research : CR 39:22 PubMed31992334

2020

Hedgehog signaling promotes sorafenib resistance in hepatocellular carcinoma patient-derived organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Wang,Yang Wang,Xiaodong Xun,Changkun Zhang,Xiao Xiang,Qian Cheng,Shihua Hu,Zhao Li,Jiye Zhu

Scientific reports 9:8401 PubMed31182739

2019

Overexpression of MAP3K3 promotes tumour growth through activation of the NF-κB signalling pathway in ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Sha-Sha Wang,Lin Tao,Li-Juan Pang,Hong Zou,Wei-Hua Liang,Zheng Liu,Su-Liang Guo,Jin-Fang Jiang,Wen-Jie Zhang,Wei Jia,Feng Li

Experimental and therapeutic medicine 12:1741-1749 PubMed27602089

2016

Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Chu,Lili Guan,Dihua Huang,Yuezhong Ren,Yan Zhou

Anatomical record (Hoboken, N.J. : 2007) 298:727-34 PubMed25388155

2014

The expression and role of MEKK3 in renal clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongsheng Lu,Xuequan Cao,Qi Chen,Lanxi Chen,Lili Chen,Meifu Gan

Asian Pacific journal of cancer prevention : APJCP 15:5271-6 PubMed25040987

2014

MEKK3 and survivin expression in cervical cancer: association with clinicopathological factors and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Quan Cao,Hong-Sheng Lu,Ling Zhang,Li-Li Chen,Mei-Fu Gan

Pigment cell & melanoma research 23:190-200 PubMed20149136

2010

PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth Halaban,Wengeng Zhang,Antonella Bacchiocchi,Elaine Cheng,Fabio Parisi,Stephan Ariyan,Michael Krauthammer,James P McCusker,Yuval Kluger,Mario Sznol

Reproduction (Cambridge, England) 136:423-32 PubMed18583472

2008

Expression profiles of protein tyrosine kinase genes in human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Young Son,Janghwan Kim,Hyo-Won Han,Sun-Mi Woo,Yee Sook Cho,Yong-Kook Kang,Yong-Mahn Han

The Journal of biological chemistry 283:13261-8 PubMed18308725

2008

Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit nuclear factor kappaB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Adi E Matitau,Michael P Scheid

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEKK3 抗体 [ep600y]

Anti-MEKK3 antibody [ep600y]

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] (AB40756)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody [ep600y] (AB40756)

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] (AB40756)

Immunocytochemistry/ Immunofluorescence - Anti-MEKK3 antibody [ep600y] (AB40756)

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] (AB40756)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (10)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise