AB154362

Anti-MEKK3 抗体

Name: Anti-MEKK3 抗体 (ab154362) | Abcam
Brand: Abcam Limited
SKU: AB154362
Price: 78000 JPY
Availability: InStock

Anti-MEKK3 antibody

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(2 Publications)

Rabbit Polyclonal MEKK3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K3 aa 250 to C-terminus.

別名を表示する

MAPKKK3, MEKK3, MAP3K3, Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded Human S-G xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling MEKK3 protein at cytoplasm and nucleus with ab154362 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling MEKK3 protein at cytoplasm and nucleus with ab154362 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-MEKK3 antibody (AB154362)

Samples were separated by 0.075 SDS-PAGE.

All lanes:

Western blot - Anti-MEKK3 antibody (ab154362) at 1/20000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

3xFlag-human MAP3K3-transfected 293T whole cell extracts at 30 µg

Predicted band size: 71 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MAP3K3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99759

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MEKK3 also known as MAP3K3 is a serine/threonine kinase that plays an important role in the MAPK signaling cascade. This protein has a molecular mass of approximately 67 kDa. MEKK3 is widely expressed with notable presence in tissues like the heart placenta and skeletal muscles. It is important for transmitting signals from the cell surface to various intracellular targets impacting numerous cellular processes.

Biological function summary

MEKK3 participates in cell growth differentiation and apoptosis regulation. It often functions within a multi-protein complex including components like MEK5 and ERK5. MEKK3 activates downstream kinases influencing gene expression and cellular responses to external signals. Its involvement is essential for controlling reactions to stress and inflammatory stimuli.

Pathways

MEKK3 integrates into the MAPK and NF-κB pathways both critical for mediating signaling responses. In the MAPK pathway MEKK3 regulates kinases like MEK5 and ERK5. Additionally it connects with proteins such as IKK in the NF-κB pathway influencing immunity and cell survival. These interactions position MEKK3 centrally in cellular defense and adaptability mechanisms.

MEKK3 associates with pathological conditions like cardiovascular diseases and cancer. Its role in these disorders links closely with dysregulation in the MAPK pathway where MEK5 and ERK5 also play significant roles. Furthermore aberrations in the NF-κB pathway involving IKK can contribute to its involvement in these diseases highlighting MEKK3's impact on disease progression and potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

See full target information MAP3K3

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Communications medicine 5:110 PubMed40216942

2025

Rescue of lysosomal acid lipase deficiency in mice by rAAV8 liver gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Laurent,Rim Harb,Christine Jenny,Julie Oustelandt,Simon Jimenez,Jeremie Cosette,Francesca Landini,Aristide Ferrante,Guillaume Corre,Nemanja Vujic,Claudia Piccoli,Anais Brassier,Laetitia Van Wittenberghe,Giuseppe Ronzitti,Dagmar Kratky,Consiglia Pacelli,Mario Amendola

International journal of molecular medicine 43:1203-1216 PubMed30628663

2019

miR‑505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT‑NFκB pathway in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Tang,Weihong Lv,Wenxin Sun,Qiaojie Bi,Yueqin Hao

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-MEKK3 抗体

Anti-MEKK3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Western blot - Anti-MEKK3 antibody (AB154362)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise