Anti-MEK4/MKK4 抗体 [EP615Y] (ab33912)
![Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)](https://www.abcam.co.jp/ps/products/33/ab33912/Images/ab33912-450824-anti-mek4-mkk4-antibody-ep615y-westernblot-brain-rat.jpg "Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP615Y] to MEK4/MKK4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MEK4/MKK4 antibody [EP615Y]
MEK4/MKK4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP615Y] to MEK4/MKK4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MEK4/MKK4 (C terminal). The exact sequence is proprietary.
Database link: P45985 -
ポジティブ･コントロール
- WB: NIH/3T3 and HeLa whole cell lysates. Rat brain tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
ab33912 is a purified IgG. -
ポリ/モノ
モノクローナル -
クローン名
EP615Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 44 kDa.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 44 kDa. |
ターゲット情報
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機能
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
組織特異性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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参照データベース
- Entrez Gene: 6416 Human
- Entrez Gene: 26398 Mouse
- Entrez Gene: 287398 Rat
- Omim: 601335 Human
- SwissProt: P45985 Human
- SwissProt: P47809 Mouse
- Unigene: 514681 Human
- Unigene: 412922 Mouse
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別名
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
画像
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Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)Anti-MEK4/MKK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (Purified) + Rat brain lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)](https://www.abcam.co.jp/ps/products/33/ab33912/Images/ab33912-450823-anti-mek4-mkk4-antibody-ep615y-westernblot-human-mouse.jpg)
Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)All lanes : Anti-MEK4/MKK4 antibody [EP615Y] (ab33912) at 1/4000 dilution (Purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)](https://www.abcam.co.jp/ps/products/33/ab33912/Images/ab33912-251573-anti-mek4-antibody-ep615y-western-blot.jpg)
Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (ab33912)Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab33912 は 10 報の論文で使用されています。
- Lu Z et al. PP2A protects podocytes against Adriamycin-induced injury and epithelial-to-mesenchymal transition via suppressing JIP4/p38-MAPK pathway. Cytotechnology 73:697-713 (2021). PubMed: 34629746
- Wang J et al. Inhibition of ASC enhances the protective role of salvianolic acid A in traumatic brain injury via inhibition of inflammation and recovery of mitochondrial function. Folia Neuropathol 59:50-66 (2021). PubMed: 33969677
- Wu X et al. Amyloid precursor protein promotes the migration and invasion of breast cancer cells by regulating the MAPK signaling pathway. Int J Mol Med 45:162-174 (2020). PubMed: 31746365
- Shang GK et al. Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles. J Cachexia Sarcopenia Muscle 11:1104-1120 (2020). PubMed: 32096609
- Li Y et al. MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1. J Breast Cancer 23:355-372 (2020). PubMed: 32908787
- Niu J et al. Overexpressed microRNA-136 works as a cancer suppressor in gallbladder cancer through suppression of JNK signaling pathway via inhibition of MAP2K4. Am J Physiol Gastrointest Liver Physiol 317:G670-G681 (2019). PubMed: 31369289
- Pinto SK et al. Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity. Am J Physiol Endocrinol Metab 313:E335-E343 (2017). PubMed: 28465283
- Lukey MJ et al. The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy. Nat Commun 7:11321 (2016). PubMed: 27089238
- Fey D et al. Signaling pathway models as biomarkers: Patient-specific simulations of JNK activity predict the survival of neuroblastoma patients. Sci Signal 8:ra130 (2015). PubMed: 26696630
- Wuestefeld T et al. A Direct in vivo RNAi screen identifies MKK4 as a key regulator of liver regeneration. Cell 153:389-401 (2013). IF, IHC ; Mouse . PubMed: 23582328