Anti-MEK3 (phospho T222) 抗体 (ab79586)
Key features and details
- Rabbit polyclonal to MEK3 (phospho T222)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MEK3 (phospho T222) antibody
MEK3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MEK3 (phospho T222) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human MEK3 (phospho T222).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab79586 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79586の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
組織特異性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
関連疾患
Note=Defects in MAP2K3 may be involved in colon cancer. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 5606 Human
- Entrez Gene: 26397 Mouse
- Entrez Gene: 303200 Rat
- Omim: 602315 Human
- SwissProt: P46734 Human
- SwissProt: O09110 Mouse
- Unigene: 514012 Human
- Unigene: 18494 Mouse
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別名
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
see all
画像
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Western blot - Anti-MEK3 (phospho T222) antibody (ab79586)All lanes : Anti-MEK3 (phospho T222) antibody (ab79586) at 1/500 dilution
Lane 1 : Jurkat cell extracts treated
with serum (20 %, 15 minutes)
Lane 2 : Jurkat cell extracts treated
with serum (20 %, 15 minutes) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 (phospho T222) antibody (ab79586)ab79586, at a 1/50 dilution, staining MEK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab79586 は 1 報の論文で使用されています。
- Ding J et al. The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction. PLoS One 18:e0286903 (2023). PubMed: 37310976