Anti-MEK3 抗体 [EP555Y] (ab40836)
![Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)](https://www.abcam.co.jp/ps/products/40/ab40836/Images/ab40836-260986-ab40836KOWB.jpg "Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP555Y] to MEK3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MEK3 antibody [EP555Y]
MEK3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP555Y] to MEK3 -
由来種
Rabbit -
特異性
This antibody is specific for MEK3.
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アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat, Hela, MCF-7 and K562 cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP555Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40836の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
|
WB
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
組織特異性
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
関連疾患
Note=Defects in MAP2K3 may be involved in colon cancer. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 5606 Human
- Omim: 602315 Human
- SwissProt: P46734 Human
- Unigene: 514012 Human
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別名
- AW212142 antibody
- dual specificity mitogen activated protein kinase kinase 3 antibody
- Dual specificity mitogen-activated protein kinase kinase 3 antibody
see all
画像
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Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)](https://www.abcam.co.jp/ps/products/40/ab40836/Images/ab40836.jpg)
Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Anti-MEK3 antibody [EP555Y] (ab40836)](https://www.abcam.co.jp/ps/products/40/ab40836/Images/ab40836-3-benefits-of-recombinant-antibodies.png)
Anti-MEK3 antibody [EP555Y] (ab40836)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab40836 は論文での使用が確認できていません。