Anti-MEK1 (phospho T286) 抗体 [EP2364Y] (ab68140)
![Western blot - Anti-MEK1 (phospho T286) antibody [EP2364Y] (ab68140)](https://www.abcam.co.jp/ps/products/68/ab68140/Images/ab68140wb.jpg "Western blot - Anti-MEK1 (phospho T286) antibody [EP2364Y] (ab68140)")
Key features and details
- Rabbit monoclonal [EP2364Y] to MEK1 (phospho T286)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-MEK1 (phospho T286) antibody [EP2364Y]
MEK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2364Y] to MEK1 (phospho T286) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: A431 cell lysate, treated with nocodazole.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2364Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68140の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 43 kDa.
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| 特記事項 |
|---|
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WB
1/2000. Predicted molecular weight: 43 kDa. |
ターゲット情報
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機能
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. -
組織特異性
Widely expressed, with extremely low levels in brain. -
関連疾患
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 5604 Human
- Omim: 176872 Human
- SwissProt: Q02750 Human
- Unigene: 145442 Human
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別名
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
- ERK activator kinase 1 antibody
see all
画像
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Western blot - Anti-MEK1 (phospho T286) antibody [EP2364Y] (ab68140)All lanes : Anti-MEK1 (phospho T286) antibody [EP2364Y] (ab68140) at 1/2000 dilution
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with nocodazole
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab68140 は論文での使用が確認できていません。