AB47295

Anti-MEK1 (phospho S217) 抗体

Name: Anti-MEK1 (phospho S217) 抗体 (ab47295) | Abcam
Brand: Abcam Limited
SKU: AB47295
Price: 76000 JPY
Availability: InStock

Anti-MEK1 (phospho S217) antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal MEK2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAP2K1 phospho S217 aa 150-250.

別名を表示する

MEK2, MKK2, PRKMK2, MAP2K2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S217) antibody (AB47295)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S217) antibody (AB47295)

ab47295, at a dilution of 1/50, staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of the immunizing phosphopeptide.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human MAP2K1 phospho S217 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q02750

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

This antibody detects endogenous levels of MEK1 only when phosphorylated at serine 217.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 also known as MAP2K1 is a dual-specificity kinase that plays a role in the MAPK/ERK pathway. It has a molecular weight of around 45 kDa. MEK1 phosphorylates and activates the extracellular signal-regulated kinases ERK1 and ERK2. MEK1 is expressed in various tissues but is especially abundant in brain and heart tissues. Researchers commonly measure MEK1 levels using techniques like MEK1 ELISA while phospho-MEK antibodies help analyze its activity state.

Biological function summary

MEK1 impacts cell processes such as proliferation differentiation and survival. It does not work alone but forms a complex with other proteins to exert its function. MEK1 activity is tightly controlled by upstream activators and downstream targets. One of the key phosphorylation sites on MEK1 is at serine 298 often marked as MEK1 pS298. This phosphorylation indicates MEK1's activated state which is critical for its biological function.

Pathways

MEK1 integrates signals within the MAPK/ERK pathway and interacts closely with RAF kinases upstream and ERK kinases downstream. This pathway modulates cellular responses to growth factors. MEK1 undergoes phosphorylation enhancing its activity and subsequently phosphorylates ERK proteins. Many studies isolate MEK1 activity using specific inhibitors such as the MEK1 inhibitor to dissect pathway dynamics. ERK inhibitor PD98059 also helps when studying parallel interactions with MEK1.

MEK1 mutations or dysregulation have links to several types of cancer including melanoma. These often involve changes that lead to continuous MEK1 activation contributing to uncontrolled cell growth. MEK1 involvement in the RASopathies also shows its connection to disorders characterized by heart and facial abnormalities. In cancer MEK1 works closely with the BRAF protein which often harbors mutations leading to disease progression showcasing MEK1 as a target for therapeutic intervention.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126).

See full target information MAP2K2

追加のターゲット

MAP2K2

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114941 PubMed39636728

2024

Myosin 1b regulates intestinal epithelial morphogenesis via interaction with UNC45A.

Applications

Unspecified application

Species

Unspecified reactive species

Céline Revenu,Corinne Lebreton,Magda Cannata Serio,Marion Rosello,Rémi Duclaux-Loras,Karine Duroure,Ophélie Nicolle,Fanny Eggeler,Marie-Thérèse Prospéri,Julie Stoufflet,Juliette Vougny,Priscilla Lépine,Grégoire Michaux,Nadine Cerf-Bensussan,Evelyne Coudrier,Franck Perez,Marianna Parlato,Filippo Del Bene

View all publications

style

left-column

style

left-column

style

right-column