Anti-MEIS1 抗体 (ab19867)

Thank you for taking the time to complete our questionnaire and contact us. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results I would also appreciate if you can confirm some further details:

1. I am sorry I am not able to trace this order number on our system, and there are no orders from the Netherlands in March or April this year. Please confirm if the product code is correct (ab19867 Anti-MEIS1 antibody - ChIP Grade) and please provide the name and address of the institution where the order was placed from. If you ordered from a purchasing agent somewhere else, then they should have the Abcam order reference number.

2. We recommend to store antibodies as directed on the datasheet so that they will remain stable and that we can continue to provide our guarantee. If addition of glycerol is required, this will be mentioned on the datasheet. For future reference, this antibody should be aliquotted and stored as it is, as directed on the datasheet. Extra glycerol is not required in this case. Storing an antibody at lower concentrations can affect its stability.

3. I can recommend to lyse the cells in RIPA lysis buffer, which should provide a more suitable protein preparation. Once the lysate is prepared, you can then add sample buffer including reducing and denaturing agents, and heat.

4. What dilutions of antibody have been tried? To reduce the background, I c an recommend to try reducing the concentration of antibody. Incubate overnight at 4oC, which can provide more specific and efficient staining.

5. I can recommend to add a wash step after the primary antibody incubation which will help to wash away any excess antibody.

6. Could you confirm the current vial of secondary antibody is working well with other primary antibodies? I suggest it would be beneficial to include a no primary control to assess if the secondary antibody is binding non specifically. It may be that the concentration of secondary antibody needs to be reduced in order to help optimize the results.

7. We recommend not to mix blocking agents in an experiment, it can sometimesaffect the results. Try 5% BSA only.

8. Has the quality of the sample and transfer to the membrane been assessed using a loading control?

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and Mouse, Human and Zebrafish samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this,it would be helpful tohave some further information and I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:


Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

DISCOUNT CODE: ****
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab19867 in Axololt. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for Axololt and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.