AB30644

Anti-MEF2A (phospho T312) 抗体

Name: Anti-MEF2A (phospho T312) 抗体 (ab30644) | Abcam
Brand: Abcam Limited
SKU: AB30644
Price: 78000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Anti-MEF2A (phospho T312) antibody

(2 Reviews)

(6 Publications)

Rabbit Polyclonal MEF2A phospho T312 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MEF2A phospho T312.

別名を表示する

MEF2, MEF2A, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1

3 Images

AbReview43157****

Western blot - Anti-MEF2A (phospho T312) antibody (AB30644)

All lanes:

Western blot - Anti-MEF2A (phospho T312) antibody (ab30644) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate - whole at 40 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A (phospho T312) antibody (AB30644)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A (phospho T312) antibody (AB30644)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma. Left : using ab30644 (10ug/ml); right : the same antibody preincubated with synthesized phosphopeptide.

Unknown

Western blot - Anti-MEF2A (phospho T312) antibody (AB30644)

All lanes:

Western blot - Anti-MEF2A (phospho T312) antibody (ab30644) at 1 µg/mL

Lane 1:

Untreated NIH/3T3 cells (5-30ug).

Lane 2:

NIH/3T3 cells treated with PMA (5-30ug)

Predicted band size: 34 kDa,55 kDa

Observed band size: 65 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human MEF2A phospho T312. The exact immunogen used to generate this antibody is proprietary information.

Q02078

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.

Biological function summary

MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.

Pathways

MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.

Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

See full target information MEF2A phospho T312

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:17364 PubMed40389489

2025

Inhibitory effects of high extracellular L-glutamate concentrations on skeletal myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Himiko Ban,Koji Nobe,Soushi Kobayashi

Redox biology 36:101669 PubMed32818796

2020

Oxidation of HDAC4 by Nox4-derived HO maintains tube formation by endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Schader,Oliver Löwe,Christina Reschke,Pedro Malacarne,Fabian Hahner,Niklas Müller,Anna Gajos-Draus,Johannes Backs,Katrin Schröder

Journal of clinical biochemistry and nutrition 61:6-13 PubMed28751803

2017

CAPE increases the expression of SOD3 through epigenetics in human retinal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Ohashi,Hiroyuki Yasuda,Tetsuro Kamiya,Hirokazu Hara,Tetsuo Adachi

The Biochemical journal 459:427-39 PubMed24517375

2014

A novel p38 MAPK docking-groove-targeted compound is a potent inhibitor of inflammatory hyperalgesia.

Applications

Unspecified application

Species

Unspecified reactive species

Hanneke L D M Willemen,Pedro M Campos,Elisa Lucas,Antonio Morreale,Rubén Gil-Redondo,Juan Agut,Florenci V González,Paula Ramos,Cobi Heijnen,Federico Mayor,Annemieke Kavelaars,Cristina Murga

Arteriosclerosis, thrombosis, and vascular biology 29:1902-8 PubMed19696400

2009

Flow activation of AMP-activated protein kinase in vascular endothelium leads to Krüppel-like factor 2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Young,Wei Wu,Wei Sun,H Benjamin Larman,Harry B Larman,Nanping Wang,Yi-Shuan Li,John Y Shyy,Shu Chien,Guillermo García-Cardeña

The Journal of neuroscience : the official journal of the Society for Neuroscience 29:6700-9 PubMed19458239

2009

A retrograde neuronal survival response: target-derived neurotrophins regulate MEF2D and bcl-w.

Applications

Species

Rat

Maria F Pazyra-Murphy,Aymeric Hans,Stephanie L Courchesne,Christoph Karch,Katharina E Cosker,Heather M Heerssen,Fiona L Watson,Taekyung Kim,Michael E Greenberg,Rosalind A Segal

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2A (phospho T312) 抗体

Anti-MEF2A (phospho T312) antibody

Western blot - Anti-MEF2A (phospho T312) antibody (AB30644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A (phospho T312) antibody (AB30644)

Western blot - Anti-MEF2A (phospho T312) antibody (AB30644)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise