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リコンビナントRabMAb

Anti-MEF2A 抗体 [EP1706Y] (ab76063)

Reviews (2) Submit a question References (9)
Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EP1706Y] (ab76063)
  • Flow Cytometry (Intracellular) - Anti-MEF2A antibody [EP1706Y] (ab76063)
  • Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
  • Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
  • Flow Cytometry (Intracellular) - Anti-MEF2A antibody [EP1706Y] (ab76063)
  • Anti-MEF2A antibody [EP1706Y] (ab76063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1706Y] to MEF2A
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-MEF2A antibody [EP1706Y]
    MEF2A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1706Y] to MEF2A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human MEF2A aa 400-500. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: Fetal brain, human heart, 3T3-L1, Mouse brain lysates, Rat brain and HeLa lysates. ICC/IF: MCF7 cells Flow Cyt (intra): MCF7 cells lysates

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76063の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC/IF
1/100 - 1/250.

ターゲット情報

  • 機能

    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.

  • 組織特異性

    Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

  • 関連疾患

    Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].

  • 配列類似性

    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.

  • 翻訳後修飾

    Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
    Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
    Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ADCAD1 antibody
    • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
    • MEF2 antibody
    • MEF2A antibody
    • MEF2A_HUMAN antibody
    • Myocyte enhancer factor 2A antibody
    • Myocyte-specific enhancer factor 2A antibody
    • RSRFC4 antibody
    • RSRFC9 antibody
    • Serum response factor like protein 1 antibody
    • Serum response factor-like protein 1 antibody
    see all

画像

  • Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
    All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 0.2 µg/ml (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 66 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MEF2A with Purified ab76063 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry (Intracellular) - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Flow Cytometry (Intracellular) - Anti-MEF2A antibody [EP1706Y] (ab76063)

    Overlay histogram showing MCF-7 cells stained with unpurified ab76063 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76063, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. 

     

     

  • Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
    All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 1/10000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Human heart lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 55 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    The observed band size may be different from the predicted molecular weight as the protein is sumoylated.
  • Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Western blot - Anti-MEF2A antibody [EP1706Y] (ab76063)Image is courtesy of an anonymous AbReview.
    All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 1/1000 dilution (Unpurified)

    All lanes : Murine cardiomyocyte whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit polyclonal HRP conjugate.

    Performed under reducing conditions.

    Predicted band size: 55 kDa


    Exposure time: 30 seconds


    Lanes from left to right: cropped MW marker ladder, 1, 2, 3, 4.

    Blocking was performed with 5% milk for 1 hour at room temperature.

    See Abreview

  • Flow Cytometry (Intracellular) - Anti-MEF2A antibody [EP1706Y] (ab76063)
    Flow Cytometry (Intracellular) - Anti-MEF2A antibody [EP1706Y] (ab76063)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MEF2A with purified ab76063 at 1/40 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Anti-MEF2A antibody [EP1706Y] (ab76063)
    Anti-MEF2A antibody [EP1706Y] (ab76063)

参考文献 (9)

ab76063 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76063 は 9 報の論文で使用されています。

  • Wang Z  et al. Landscape of enhancer disruption and functional screen in melanoma cells. Genome Biol 24:248 (2023). PubMed: 37904237
  • Wei D  et al. Tissue Expression Analysis, Cloning, and Characterization of the 5'-Regulatory Region of the Bovine LATS1 Gene. Front Vet Sci 9:853819 (2022). PubMed: 35692290
  • Barker SJ  et al. MEF2 is a key regulator of cognitive potential and confers resilience to neurodegeneration. Sci Transl Med 13:eabd7695 (2021). PubMed: 34731014
  • Cilenti F  et al. A PGE2-MEF2A axis enables context-dependent control of inflammatory gene expression. Immunity 54:1665-1682.e14 (2021). PubMed: 34129840
  • Cai Q  et al. Inflammation-Associated Senescence Promotes Helicobacter pylori-Induced Atrophic Gastritis. Cell Mol Gastroenterol Hepatol 11:857-880 (2021). PubMed: 33161156
  • Yin JW  et al. Dexmedetomidine and Netrin-1 Combination Therapy Inhibits Endoplasmic Reticulum Stress by Regulating the ERK5/MEF2A Pathway to Attenuate Cerebral Ischemia Injury. Front Neurosci 15:641345 (2021). PubMed: 33584197
  • Estes ML  et al. Baseline immunoreactivity before pregnancy and poly(I:C) dose combine to dictate susceptibility and resilience of offspring to maternal immune activation. Brain Behav Immun 88:619-630 (2020). PubMed: 32335198
  • Belian E  et al. Forward Programming of Cardiac Stem Cells by Homogeneous Transduction with MYOCD plus TBX5. PLoS One 10:e0125384 (2015). WB, IF . PubMed: 26047103
  • Malek MH  et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013). PubMed: 23180811

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-MEF2A antibody [EP1706Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (15% PAGE)
Loading amount
25 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 05 2015

Western blot abreview for Anti-MEF2A antibody [EP1706Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 28 2015

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