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AB236399

Anti-MED9 抗体 [OTI7F11]

Anti-MED9 antibody [OTI7F11]

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(1 Publication)

Mouse Monoclonal MED9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MED9.

別名を表示する

MED25, MED9, Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (AB236399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (AB236399)

Paraffin-embedded human prostate carcinoma tissue stained for MED9 using ab236399 at 1/1000 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (AB236399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED9 antibody [OTI7F11] (AB236399)

Paraffin-embedded human breast tissue stained for MED9 using ab236399 at 1/1000 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.

Western blot - Anti-MED9 antibody [OTI7F11] (AB236399)
  • WB

Supplier Data

Western blot - Anti-MED9 antibody [OTI7F11] (AB236399)

All lanes:

Western blot - Anti-MED9 antibody [OTI7F11] (ab236399) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY MED9 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 16 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

OTI7F11

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human MED9.

Q9NWA0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
Purified from cell culture supernatant.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MED9 also known as mediator complex subunit 9 is a component of the larger mediator complex involved in transcriptional regulation. It plays a mechanical role in bridging transcriptional regulatory proteins to the RNA polymerase II enzyme. MED9 exhibits a molecular mass of approximately 11 kDa and is expressed widely in human tissue with notably higher levels in heart and skeletal muscle.
Biological function summary

MED9 is an integral part of the mediator complex which is essential for the transcription of nearly all RNA polymerase II-dependent genes. The mediator complex including MED9 facilitates communication between gene-specific regulatory proteins and the polymerase machinery which is important for transcription initiation. MED9 participates in the regulation of multiple genes impacting signal transduction pathways and influencing gene expression.

Pathways

The involvement of MED9 extends to significant biological processes including the MAPK signaling pathway and the Wnt signaling pathway. In these pathways MED9 works alongside other mediator subunits such as MED1 and MED12 to coordinate transcriptional activation. Through these interactions MED9 contributes to the regulation of genes essential for cell growth division and differentiation.

Dysregulation of MED9 has been linked to cancer and cardiac conditions. For instance aberrant MED9 activity may influence oncogenic pathways leading to uncontrolled cell proliferation. In cardiac disorders its interaction with other mediator complex subunits like MED13 and MED14 could result in impaired cardiac function and congenital heart defects. Understanding MED9's role in these contexts can help in designing targeted therapeutic strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED9
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:3807-3825 PubMed37166429

2023

Identification and validation of metabolism-related genes signature and immune infiltration landscape of rheumatoid arthritis based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Guo,Yuanye Ma,Yaqing Wang,Hongfei Xiang,Huifei Cui,Zuoran Fan,Youfu Zhu,Dongming Xing,Bohua Chen,Hao Tao,Zhu Guo,Xiaolin Wu
View all publications
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Abcam product promise

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