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リコンビナントRabMAb

Anti-MED7 抗体 [EPR15410] (ab187146)

Submit a review Submit a question References (3)
Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
  • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
  • Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
  • Anti-MED7 antibody [EPR15410] (ab187146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15410] to MED7
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-MED7 antibody [EPR15410]
    MED7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15410] to MED7

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, 293T, C6, Raw264.7, PC12 and NIH3T3 cell lysates; Human thyroid carcinoma and Mouse brain tissue; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187146の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

  • 配列類似性

    Belongs to the Mediator complex subunit 7 family.

  • 翻訳後修飾

    Constitutively sumoylated.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activator recruited cofactor 34 kDa component antibody
      • Activator-recruited cofactor 34 kDa component antibody
      • ARC34 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 9 antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9 (33kD) antibody
      • Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa antibody
      • CRSP complex subunit 9 antibody
      • CRSP33 antibody
      • CRSP9 antibody
      • hMED7 antibody
      • MED7 antibody
      • MED7_HUMAN antibody
      • Mediator complex subunit 7 antibody
      • Mediator of RNA polymerase II transcription subunit 7 antibody
      • MGC12284 antibody
      • RNA polymerase transcriptional regulation mediator subunit 7 homolog antibody
      • Transcriptional coactivator CRSP33 antibody
      see all

    画像

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      Western blot - Anti-MED7 antibody [EPR15410] (ab187146)
      All lanes : Anti-MED7 antibody [EPR15410] (ab187146) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : RAW 264.7 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 27 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)
      Flow Cytometry (Intracellular) - Anti-MED7 antibody [EPR15410] (ab187146)

      Intracellular flow cytometrical analysis of HeLa cells labeling MED7 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED7 antibody [EPR15410] (ab187146)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling MED7 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-MED7 antibody [EPR15410] (ab187146)
      Anti-MED7 antibody [EPR15410] (ab187146)

    参考文献 (3)

    ab187146 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187146 は 3 報の論文で使用されています。

    • Jackson LM  et al. Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells. Nucleic Acids Res 49:12855-12869 (2021). PubMed: 34871431
    • Schleicher EM  et al. Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. PLoS Genet 16:e1009176 (2020). PubMed: 33137164
    • Joseph C  et al. Mediator complex (MED) 7: a biomarker associated with good prognosis in invasive breast cancer, especially ER+ luminal subtypes. Br J Cancer 118:1142-1151 (2018). PubMed: 29588513

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