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AB228887

Anti-MED18 抗体

Anti-MED18 antibody

  • JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal MED18 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MED18.

別名を表示する

Mediator of RNA polymerase II transcription subunit 18, Mediator complex subunit 18, p28b, MED18

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MED18 antibody (AB228887)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MED18 antibody (AB228887)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MED18 (green) using ab228887 at 1/200 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED18 antibody (AB228887)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED18 antibody (AB228887)

Paraffin-embedded Cal27 xenograft tissue stained for MED18 protein using ab228887 at 1/500 in immunohistochemical analysis.

Western blot - Anti-MED18 antibody (AB228887)
  • WB

Supplier Data

Western blot - Anti-MED18 antibody (AB228887)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-MED18 antibody (ab228887) at 1/1000 dilution

All lanes:

A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 24 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MED18. The exact immunogen used to generate this antibody is proprietary information.

Q9BUE0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MED18 also known as Mediator Complex Subunit 18 is a component of the mediator complex. This protein possesses a mass of approximately 26 kDa. MED18 is expressed in various tissues playing a role in transcription regulation. It serves as a bridge linking transcription factors with RNA polymerase II aiding in the control of gene expression.
Biological function summary

MED18 operates as part of the larger mediator complex which is essential in conveying signals from gene-specific regulatory proteins to the transcriptional machinery. The mediator complex a multiprotein structure acts as a platform for the assembly of transcription factors and cofactors. Through its role in this complex MED18 directly influences RNA synthesis and gene transcription processes.

Pathways

MED18 is involved in critical biological pathways like the transcription initiation pathway and RNA polymerase II transcription machinery. The mediator complex where MED18 functions interacts closely with the pre-initiation complex. Proteins like MED1 and MED20 may have interactions with MED18 within these pathways contributing to its essential role in transcription regulation.

MED18 has associations with certain cancers and neurological disorders due to its role in transcription misregulation. Aberrant expression or mutations in MED18 can impact cell growth and differentiation leading to tumor development. Additionally disturbances in MED18 function can link to MED1 as both are parts of the mediator complex contributing to disruptions in normal transcription regulation that could drive disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED18
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:1062695 PubMed36568562

2022

Large-conductance Ca-activated K channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meili Wang,Shuanglei Li,Hongshan Liu,Mingyuan Liu,Jin Zhang,Yang Wu,Cangsong Xiao,Haixia Huang
View all publications
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