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リコンビナントRabMAb

Anti-MED12 抗体 [EPR25211-36] (BSA and Azide free) (ab300155)

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Western blot - Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
  • Western blot - Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
  • Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25211-36] to MED12 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-MED12 antibody [EPR25211-36] (BSA and Azide free)
    MED12 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25211-36] to MED12 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: DLD-1, LoVo, MCF7, SK-BR-3, HT-29, HCT116, Caco-2, NIH/3T3, RAW264.7, C2C12, C6, PC12; MCF7 whole cell fresh lysate. Mouse uterus tissue lysate.

  • 特記事項

    Ab300155 is a carrier free version of ab300154. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300155の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 243 kDa.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 243 kDa.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.

  • 組織特異性

    Ubiquitous.

  • 関連疾患

    Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
    Defects in MED12 are the cause of Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root.

  • 配列類似性

    Belongs to the Mediator complex subunit 12 family.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activator recruited cofactor 240 kDa component antibody
      • Activator-recruited cofactor 240 kDa component antibody
      • ARC240 antibody
      • CAG repeat protein 45 antibody
      • CAGH45 antibody
      • HOPA antibody
      • KIAA0192 antibody
      • MED12 antibody
      • MED12_HUMAN antibody
      • Mediator complex subunit 12 antibody
      • Mediator of RNA polymerase II transcription subunit 12 antibody
      • OPA containing protein antibody
      • OPA-containing protein antibody
      • Thyroid hormone receptor associated protein complex 230 kDa component antibody
      • Thyroid hormone receptor-associated protein complex 230 kDa component antibody
      • TNRC11 antibody
      • Trap230 antibody
      • Trinucleotide repeat containing gene 11 protein antibody
      • Trinucleotide repeat-containing gene 11 protein antibody
      see all

    画像

    • Western blot - Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
      Western blot - Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
      All lanes : Anti-MED12 antibody [EPR25211-36] (ab300154) at 1/1000 dilution

      Lane 1 : DLD-1 (human colorectal adenocarcinoma cell) whole cell lysate
      Lane 2 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 5 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 6 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 7 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 8 : MCF7 whole cell fresh lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 243 kDa
      Observed band size: 240 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab300154, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:28183795; PMID: 29440396 ).

      Low expression: SK-BR-3, HT-29, HCT116, Caco-2 (PMID:28183795 )

      The lysates of lane 8 were freshly made and used for Western blotting immediately to minimize protein degradation.

      The lower bands in lane 3 and lane 5 may be caused by degradation.

    • Western blot - Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
      Western blot - Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
      All lanes : Anti-MED12 antibody [EPR25211-36] (ab300154) at 1/1000 dilution

      Lane 1 : Mouse uterus tissue lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 4 : C2C12 (mouse myoblast) whole cell lysate
      Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
      Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 243 kDa
      Observed band size: 240 kDa why is the actual band size different from the predicted?



      This data was developed using ab300154, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 

      Lane 1: 59 seconds

      Lane 2-6: 15 seconds. 

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:28183795; PMID: 29440396 ).

      The lower bands may be caused by degradation.

    • Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)
      Anti-MED12 antibody [EPR25211-36] (BSA and Azide free) (AB300155)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300155 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300155 は論文での使用が確認できていません。

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