Anti-MED12 抗体 [EPR25211-36] (ab300154)
![Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)](https://www.abcam.co.jp/ps/products/300/ab300154/Images/AB300154-485715-ab300154WB1.jpg "Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25211-36] to MED12
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MED12 antibody [EPR25211-36]
MED12 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25211-36] to MED12 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
ポジティブ･コントロール
- WB: Whole cell lysates: DLD-1, LoVo, MCF7, SK-BR-3, HT-29, HCT116, Caco-2, NIH/3T3, RAW264.7, C2C12, C6, PC12; MCF7 whole cell fresh lysate. Mouse uterus tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25211-36 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300154の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 243 kDa.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 243 kDa. |
ターゲット情報
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機能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. -
組織特異性
Ubiquitous. -
関連疾患
Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Defects in MED12 are the cause of Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root. -
配列類似性
Belongs to the Mediator complex subunit 12 family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9968 Human
- Entrez Gene: 59024 Mouse
- Entrez Gene: 679693 Rat
- Omim: 300188 Human
- SwissProt: Q93074 Human
- SwissProt: A2AGH6 Mouse
- Unigene: 409226 Human
- Unigene: 20873 Mouse
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別名
- Activator recruited cofactor 240 kDa component antibody
- Activator-recruited cofactor 240 kDa component antibody
- ARC240 antibody
see all
画像
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Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)All lanes : Anti-MED12 antibody [EPR25211-36] (ab300154) at 1/1000 dilution
Lane 1 : DLD-1 (human colorectal adenocarcinoma cell) whole cell lysate
Lane 2 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 7 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 8 : MCF7 whole cell fresh lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 243 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:28183795; PMID: 29440396 ).
Low expression: SK-BR-3, HT-29, HCT116, Caco-2 (PMID:28183795 )
The lysates of lane 8 were freshly made and used for Western blotting immediately to minimize protein degradation.
The lower bands in lane 3 and lane 5 may be caused by degradation. -
![Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)](https://www.abcam.co.jp/ps/products/300/ab300154/Images/AB300154-485718-ab300154WB2.jpg)
Western blot - Anti-MED12 antibody [EPR25211-36] (AB300154)All lanes : Anti-MED12 antibody [EPR25211-36] (ab300154) at 1/1000 dilution
Lane 1 : Mouse uterus tissue lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : C2C12 (mouse myoblast) whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 243 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 59 seconds
Lane 2-6: 15 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:28183795; PMID: 29440396 ).
The lower bands may be caused by degradation. -
![Anti-MED12 antibody [EPR25211-36] (AB300154)](https://www.abcam.co.jp/ps/products/300/ab300154/Images/AB300154-485669-Benefits-of-Recombinant-antibodies.jpg)
Anti-MED12 antibody [EPR25211-36] (AB300154)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300154 は論文での使用が確認できていません。