AB254707

Anti-MECR 抗体

Name: Anti-MECR 抗体 (ab254707) | Abcam
Brand: Abcam Limited
SKU: AB254707
Price: 93000 JPY
Availability: InStock

Anti-MECR antibody

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(2 Publications)

Rabbit Polyclonal MECR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MECR aa 250 to C-terminus.

別名を表示する

NBRF1, CGI-63, MECR, 2-enoyl thioester reductase, Nuclear receptor-binding factor 1, HsNrbf-1, NRBF-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human colon tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human testis tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MECR antibody (AB254707)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MECR antibody (AB254707)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling MECR using ab254707 at 4µg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human cerebral cortex tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Paraffin-embedded human kidney tissue stained for MECR using ab254707 at 1/20 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MECR antibody (AB254707)

All lanes:

Western blot - Anti-MECR antibody (ab254707) at 0.4 µg/mL

Lane 1:

RT4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-MECR antibody (AB254707)

All lanes:

Western blot - Anti-MECR antibody (ab254707) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 40 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MECR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BV79

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MECR or mitochondrial trans-2-enoyl-CoA reductase plays an important role in mitochondrial fatty acid synthesis. This enzyme is involved in the final reduction step of trans-2-enoyl-CoA to acyl-CoA. Known alternately as NRBF1 MECR has a molecular mass of approximately 37 kDa. It finds expression primarily in mitochondria-rich tissues like heart liver and skeletal muscle where energy production and fatty acid metabolism are highly active.

Biological function summary

The MECR protein contributes to the biosynthesis of mitochondrial fatty acids by catalyzing the reduction of trans-2-enoyl-CoA. It operates as part of a multicomponent enzyme complex involved in mitochondrial fatty acid synthesis. This process is necessary for maintaining proper mitochondrial membrane integrity and function affecting multiple cellular functions beyond lipid metabolism including regulation of energy homeostasis.

Pathways

MECR integrates mainly into mitochondrial fatty acid synthesis pathways. It collaborates with other enzymes to regulate fatty acid metabolism contributing to energy production within mitochondria. MECR partners with the protein ETFDH which transfers electrons necessary for the enzymatic reactions within the pathway. Further it interacts indirectly with the beta-oxidation pathway which breaks down fatty acids into acyl-CoA units subsequently entering the mitochondrial electron transport chain for ATP production.

Defective MECR activity has been linked to progressive neurological disorders and cardiomyopathy. Disorders like mitochondrial myopathy are associated with mutations affecting MECR's enzymatic efficiency. Other proteins such as ACAD9 connect with MECR where both participate in similar or adjacent pathways disrupted in these disorders. This link emphasizes the potential role of MECR in therapeutic strategies targeting mitochondrial dysfunction-related diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed : 12654921, PubMed : 18479707, PubMed : 27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed : 37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed : 37653044). Required for maintenance of neurons (By similarity).

See full target information MECR

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39859268

2025

Identification of Downregulated Gene in Parkinson's Disease Through Integrated Transcriptomic Analysis and Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Danlei Wang,Haoheng Yu,Yi Qu,Ke An,Hongming Liang,Zhijuan Mao,Jingyi Li,Yongjie Xiong,Zhe Min,Zheng Xue

Aging cell 23:e14320 PubMed39158018

2024

Single-cell RNA sequencing reveals the CRTAC1 population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yulong Tang,Dachun Zhuo,Yuexin Yu,Weilin Pu,Yanyun Ma,Yuting Zhang,Yan Huang,Qing Zhang,Kunhai Tang,Chen Meng,Di Yang,Lu Bai,Dongyi He,Li Jin,Hejian Zou,Huji Xu,Qi Zhu,Jiucun Wang,Yuanyuan Chen,Jing Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MECR 抗体

Anti-MECR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Immunocytochemistry/ Immunofluorescence - Anti-MECR antibody (AB254707)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

Western blot - Anti-MECR antibody (AB254707)

Western blot - Anti-MECR antibody (AB254707)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise