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Anti-Measles nucleoprotein 抗体 [3E1] (ab9397)

Submit a review Q&A (1)References (7)
Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)

    Key features and details

    • Mouse monoclonal [3E1] to Measles nucleoprotein
    • Suitable for: WB
    • Reacts with: Species independent
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Measles nucleoprotein antibody [3E1]
      Measles nucleoprotein 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [3E1] to Measles nucleoprotein

    • 由来種

      Mouse

    • 特異性

      Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Recombinant full length protein corresponding to Measles virus Measles nucleoprotein.

    • ポジティブ･コントロール

      • recombinant measles NP

    • 特記事項

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR210126-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.2
      Preservative: 0.1% Sodium azide
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      3E1

    • ミエローマ

      NS0/1

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9397の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000 - 1/5000.
    特記事項
    WB
    1/1000 - 1/5000.

    ターゲット情報

    • 関連性

      Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest

    • 細胞内局在

      Virion. Host cytoplasm.

    • 別名

      • Measles nucleocapsid protein antibody
      • N antibody
      • NP antibody
      • Nucleocapsid protein antibody

    画像

    • Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)
      Western blot - Anti-Measles nucleoprotein antibody [3E1] (ab9397)

      Immunoblot of measles NP using monoclonal antibody 3E1.
      Antibody 3E1 at a concentration of 0.5 µg/ml.
      Lane 1 - prestained marker proteins
      lane 2 - recombinant measles NP.

      Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.

    参考文献 (7)

    ab9397 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9397 は 7 報の論文で使用されています。

    • Collados Rodríguez M  et al. Novel Antiviral Molecules against Ebola Virus Infection. Int J Mol Sci 24:N/A (2023). PubMed: 37834238
    • Frantz PN  et al. A live measles-vectored COVID-19 vaccine induces strong immunity and protection from SARS-CoV-2 challenge in mice and hamsters. Nat Commun 12:6277 (2021). PubMed: 34725327
    • Sun Q  et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res 29:90-102 (2014). PubMed: 23794264
    • Davis ME  et al. Antagonism of the phosphatase PP1 by the measles virus V protein is required for innate immune escape of MDA5. Cell Host Microbe 16:19-30 (2014). WB . PubMed: 25011105
    • Wang FM  et al. Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signaling. Bone 53:269-76 (2013). PubMed: 23262029
    • Ito M  et al. Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1. J Virol 87:9633-42 (2013). PubMed: 23804634
    • Sharma LB  et al. Contribution of matrix, fusion, hemagglutinin, and large protein genes of the CAM-70 measles virus vaccine strain to efficient growth in chicken embryonic fibroblasts. J Virol 83:11645-54 (2009). WB . PubMed: 19726516

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Do you have antibody for "Measles virus nucleocapsid"? I like to know whether Nucleoprotein and Nucleocapsids are same? If so which of your product is best cross reacted for western application among the following: ab9882, ab9397, ab9884, ab9885 .

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    Answer

    Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and ribosomes, and in viruses. A nucleocapsid is a unit of viral structure, consisting of a capsid (protein coat) with the enclosed nucleic acid; some simple viruses are naked nucleocapsids, while in others the nucleocapsids form part of a more complex structure. According to the database Expasy.org (http://www.expasy.org/cgi-bin/get-entries?KW=Viral%20nucleoprotein),a viral nucleocapsid protein is a synonym of a viral nucleoprotein therefore indeed they are the same. I have looked at the datasheets of the four antibodies you are interested in and I would recommend looking in particular at the datasheets of ab9882 and ab9397 as both have been tested in WB and therefore are guaranteed to work in this application. Ab9884 also works in WB but at a lower dilution so you will need to use more antibody. Ab9885 has not been tested in WB, only in ICC so is not guaranteed to work in WB. I hope this information will help you choose a suitable antibody for your experiments, please do not hesitate to contact me again if I can be of further help,

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