Anti-Measles nucleoprotein 抗体 [2D7] (ab9882)
Key features and details
- Mouse monoclonal [2D7] to Measles nucleoprotein
- Suitable for: ELISA, WB
- Isotype: IgG1
製品の概要
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製品名
Anti-Measles nucleoprotein antibody [2D7]
Measles nucleoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2D7] to Measles nucleoprotein -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Measles virus -
免疫原
Recombinant full length protein corresponding to Measles virus Measles nucleoprotein. Recombinant measles NP
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ポジティブ・コントロール
- recombinant measles NP
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特記事項
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
2D7 -
ミエローマ
NS0/1 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9882の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/1000 - 1/10000.
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WB |
1/500 - 1/2000. Detects a band of approximately 52.5 kDa.
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特記事項 |
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ELISA
1/1000 - 1/10000. |
WB
1/500 - 1/2000. Detects a band of approximately 52.5 kDa. |
ターゲット情報
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関連性
Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest -
細胞内局在
Virion. Host cytoplasm. -
別名
- Measles nucleocapsid protein antibody
- N antibody
- NP antibody
- Nucleocapsid protein antibody
画像
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Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
Lane 2 - recombinant measles NP.Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
Lane 2 - recombinant measles NP.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab9882 は 5 報の論文で使用されています。
- Lo MK et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). PubMed: 28262699
- Morin I & Schaeffer PM Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein. Anal Biochem 420:121-6 (2012). PubMed: 21971444
- Morin I et al. IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform. Analyst 136:4815-21 (2011). PubMed: 21980595
- Kurihara N et al. Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. Cell Metab 13:23-34 (2011). IHC ; Human . PubMed: 21195346
- Zvirbliene A et al. Mapping of B cell epitopes in measles virus nucleocapsid protein. Arch Virol 152:25-39 (2007). PubMed: 16944047