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Anti-MDMX/MDM4 抗体 (ab16058)

Reviews (1)Q&A (1)References (11)
Immunocytochemistry/ Immunofluorescence - Anti-MDMX/MDM4 antibody (ab16058)
  • Western blot - Anti-MDMX/MDM4 antibody (ab16058)

Key features and details

  • Rabbit polyclonal to MDMX/MDM4
  • Suitable for: ICC/IF, WB
  • Reacts with: Cow, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-MDMX/MDM4 antibody
    MDMX/MDM4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to MDMX/MDM4

  • 由来種

    Rabbit

  • 特異性

    Two bands were detected with the antibody in HeLa nuclear extract, at ~80 and 100 kDa. The 80 kDa band was no longer detected after the addition of competing peptide, suggesting that this band corresponds to MDMX/MDM4. MDMX/MDM4 has been shown previously to migrate aberrantly at an ~80 kDa size (see deGraaf, et.al., J Biol Chem. 2003 Oct 3;278(40):38315-24). The 100 kDa band is not competed away by the addition of the immunizing peptide, suggesting that it is nonspecific cross-reactivity.

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Cow, Human

  • 免疫原

    Synthetic peptide corresponding to Human MDMX/MDM4 aa 200-300 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16383)

  • ポジティブ･コントロール

    • Recombinant Human MDMX/MDM4 protein (ab114467) can be used as a positive control in WB. This antibody gave a positive signal in the following nuclear cell extract: HeLa. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa. ICC/IF: HeLa cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16058の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
Use a concentration of 5 µg/ml.
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 58 kDa).
特記事項
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 機能

    Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.

  • 組織特異性

    Expressed in all tissues tested with high levels in thymus.

  • 配列類似性

    Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.

  • ドメイン

    Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.

  • 翻訳後修飾

    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp781B1423 antibody
      • Double minute 4 antibody
      • Double minute 4 human homolog of p53 binding protein antibody
      • Double minute 4 protein antibody
      • HDMX antibody
      • MDM 4 antibody
      • Mdm2 like p53 binding protein antibody
      • Mdm2-like p53-binding protein antibody
      • MDM4 antibody
      • Mdm4 p53 binding protein homolog mouse antibody
      • Mdm4 protein antibody
      • MDM4 related protein 1 antibody
      • Mdm4 transformed 3T3 cell double minute 4 antibody
      • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
      • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
      • MDM4_HUMAN antibody
      • Mdmx protein antibody
      • MGC132766 antibody
      • Mouse double minute 4 homolog antibody
      • Mouse double minute 4 human homolog of p53 binding protein antibody
      • MRP 1 antibody
      • MRP1 antibody
      • p53 binding protein antibody
      • p53 BINDING PROTEIN MDM4 antibody
      • p53-binding protein Mdm4 antibody
      • Protein Mdm4 antibody
      • Protein Mdmx antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-MDMX/MDM4 antibody (ab16058)
      Immunocytochemistry/ Immunofluorescence - Anti-MDMX/MDM4 antibody (ab16058)
      ab16058 staining MDMX/MDM4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16058 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
    • Western blot - Anti-MDMX/MDM4 antibody (ab16058)
      Western blot - Anti-MDMX/MDM4 antibody (ab16058)
      All lanes : Anti-MDMX/MDM4 antibody (ab16058) at 1/1000 dilution

      Lane 1 : 20ug calf thymus histone
      Lane 2 : 20ug HeLa nuclear extract
      Lane 3 : 20ug calf thymus histone with Human MDMX/MDM4 peptide (ab16383) at 1 µg/ml
      Lane 4 : 20ug HeLa nuclear extract with Human MDMX/MDM4 peptide (ab16383) at 1 µg/ml

      Secondary
      All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 58 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa (possible cross reactivity)



      Two bands were detected with the antibody in HeLa nuclear extract, at ~80 and 100 kDa. The 80 kDa band was no longer detected after the addition of competing peptide, suggesting that this band corresponds to MDMX/MDM4. The 100 kDa band is not competed away by the addition of the immunizing peptide, suggesting that it is nonspecific cross-reactivity.

    参考文献 (11)

    ab16058 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16058 は 11 報の論文で使用されています。

    • Uddin MA  et al. Hsp90 inhibition protects brain endothelial cells against LPS-induced injury. Biofactors 48:926-933 (2022). PubMed: 35266593
    • Kubra KT & Barabutis N P53 in endothelial function and unfolded protein response regulation. Cell Biol Int 46:2257-2261 (2022). PubMed: 35998257
    • Elmarakeby HA  et al. Biologically informed deep neural network for prostate cancer discovery. Nature 598:348-352 (2021). PubMed: 34552244
    • Sahin I  et al. Hyperprogression of a mismatch repair-deficient colon cancer in a humanized mouse model following administration of immune checkpoint inhibitor pembrolizumab. Oncotarget 12:2131-2146 (2021). PubMed: 34676046
    • Fan Y  et al. Recombinant Dual-target MDM2/MDMX Inhibitor Reverses Doxorubicin Resistance through Activation of the TAB1/TAK1/p38 MAPK Pathway in Wild-type p53 Multidrug-resistant Breast Cancer Cells. J Cancer 11:25-40 (2020). PubMed: 31892970
    View all Publications for this product

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MDMX/MDM4 antibody

     Average
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (fibroblast)
    Specification
    fibroblast
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Jesus Espada

    Verified customer

    投稿 Jul 13 2006

    Question

    It is written that the band should be around 55kDa on the ab49993 data sheet, but the band on the western blotting of the ab16058 is around 80kDa. Why the actual band size is different from the predicted size?

    Read More
    Answer

    Thank you for your inquiry. The actual size was smaller than the predicted one, the reason can be thought that the post translational modification, e.g. phosphorylation, glycosylation etc. They increase the size of the protein. The 80 kDa band of ab16058 datasheet was no longer detected after the addition of competing peptide, suggesting that this band corresponds to MDMX.

    Read More

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