Anti-MDC1 抗体 [MDC1-50] (ab50003)
Key features and details
- Mouse monoclonal [MDC1-50] to MDC1
- Suitable for: ICC, IP, ICC/IF, WB
- Reacts with: Human, Monkey
- Isotype: IgG2a
製品の概要
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製品名
Anti-MDC1 antibody [MDC1-50]
MDC1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MDC1-50] to MDC1 -
由来種
Mouse -
アプリケーション
適用あり: ICC, IP, ICC/IF, WBmore details -
種交差性
交差種: Human, Monkey -
免疫原
Recombinant fragment, corresponding to amino acids 2-200 of Human MDC1
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ポジティブ・コントロール
- WB: total cell extract of G361 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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特記事項(精製)
This antibody is a purified Mouse Immunoglobulin. -
ポリ/モノ
モノクローナル -
クローン名
MDC1-50 -
ミエローマ
NS1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50003の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. -
組織特異性
Highly expressed in testis. -
配列類似性
Contains 2 BRCT domains.
Contains 1 FHA domain. -
ドメイン
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX. -
翻訳後修飾
Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
細胞内局在
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. - Information by UniProt
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参照データベース
- Entrez Gene: 9656 Human
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- Unigene: 653495 Human
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別名
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab50003 は 16 報の論文で使用されています。
- Gelot C et al. Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis. Nature 621:415-422 (2023). PubMed: 37674080
- Lee KY & Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A. Cell Rep 34:108680 (2021). PubMed: 33503415
- Ka NL et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Cell Rep 37:110138 (2021). PubMed: 34936865
- Bergstrand S et al. Biallelic mutations in WRAP53 result in dysfunctional telomeres, Cajal bodies and DNA repair, thereby causing Hoyeraal-Hreidarsson syndrome. Cell Death Dis 11:238 (2020). PubMed: 32303682
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317