AB50003

Anti-MDC1 抗体 [MDC1-50]

Name: Anti-MDC1 抗体 [MDC1-50] (ab50003) | Abcam
Brand: Abcam Limited
SKU: AB50003
Price: 63000 JPY
Availability: InStock
Rating: 4 (3 reviews)

Anti-MDC1 antibody [MDC1-50]

(3 Reviews)

(18 Publications)

Mouse Monoclonal MDC1 antibody. Suitable for ICC, IP, WB, ICC/IF and reacts with Monkey, Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDC1 aa 1-200.

別名を表示する

KIAA0170, NFBD1, MDC1, Mediator of DNA damage checkpoint protein 1, Nuclear factor with BRCT domains 1

1 Images

CiteAb

Western blot - Anti-MDC1 antibody [MDC1-50] (AB50003)

Western Blotting using Anti-MDC1 antibody [MDC1-50], ab50003. Publication image from Farnebo, M. et al., 2016, Cell Death Differ, 27315300. Legend direct from paper.

Inhibition of splicing downregulates repair factors at both the mRNA and protein levels. (a) U2OS cells were treated with pladienolide B for 2, 6 and 16 h and irradiated (6 Gy, 1 h recovery) 1 h prior to termination of the treatment. The levels of the indicated mRNAs were measured through qPCR analysis. The change is relative to the DMSO control and two reference genes (18S rRNA and β-actin). Means±S.D. are shown, n=3. (b) qPCR analysis of the levels mRNA and pre-mRNA for RNF8 and RAD51 in U2OS cells treated with pladienolide B or isoginkgetin for 2, 6 or 16 h and irradiated (6 Gy, 1 h recovery) 1 h prior to termination of this treatment. The change is expressed relative to the DMSO control value and the levels of mRNA for two reference genes (18S rRNA and β-actin). Means±S.D. are shown, n=3. The arrows indicate the positioning of the PCR primers used and their sequences are shown in Supplementary Table S1. (c) Western blotting following pladienolide B treatment of U2OS cells as described in (a). β-Actin was used as a loading control. Densitometric quantification of each protein is shown in Supplementary Figure S2B. The three MDC1 bands correspond to the unphosphorylated, phosphorylated and hyperphosphorylated forms of full-length MDC1

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MDC1-50

アイソタイプ

IgG2a

キャリアフリー

交差種

Human, Monkey

アプリケーション

ICC, ICC/IF, IP, WB

applications

免疫原

Recombinant Fragment Protein within Human MDC1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q14676

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

This antibody is a purified Mouse Immunoglobulin.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MDC1 also known as mediators of DNA damage checkpoint protein 1 or ABF-M 1711 is an important player in the DNA damage response (DDR). It has a mass of approximately 220 kDa. MDC1 is highly expressed in various human tissues particularly where cell turnover is high like in the bone marrow and lymphoid organs. In cellular operations MDC1 serves as a scaffold protein that coordinates recruitment and activation of DDR machinery at sites of double-strand breaks (DSBs) on DNA.

Biological function summary

This protein plays a vital role in maintaining genomic stability by binding to phosphorylated histone H2AX at DSB sites. It is not a solitary player; MDC1 functions as part of a larger protein complex including factors like RNF8 RNF168 and 53BP1. This complex is essential for amplifying the DDR signal and facilitating the repair process through non-homologous end joining (NHEJ) and homologous recombination (HR). MDC1's interaction with other repair proteins helps to extend the signal required for effective DNA repair.

Pathways

MDC1 significantly influences cellular pathways involving DNA damage sensing and repair and cell cycle checkpoints. It ties closely to the ATM kinase pathway which is activated in response to DSBs. MDC1 aids ATM in phosphorylating downstream targets like CHK2 and p53 for cell cycle arrest. It also connects with BRCA1 interacting in HR repair pathways to ensure accurate repair of DSBs. Both ATM and BRCA1 interactions illustrate MDC1's pivotal role in maintaining DNA integrity.

MDC1 expression and functionality have profound implications on cancer and immunodeficiencies. Abnormal MDC1 expression or mutations can lead to impaired DNA repair and genomic instability often associated with tumor progression in cancers such as breast cancer. Moreover MDC1 interacts with proteins like p53 which is a well-known tumor suppressor to hinder cancer development by stopping the cell cycle for repair or triggering apoptosis if the damage is beyond repair. Dysregulation in MDC1-related pathways can also compromise immune system effectiveness further underpinning its significance in disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle (PubMed : 12475977, PubMed : 12499369, PubMed : 12551934, PubMed : 12607003, PubMed : 12607004, PubMed : 12607005, PubMed : 12611903, PubMed : 14695167, PubMed : 15201865, PubMed : 15377652, PubMed : 16049003, PubMed : 16377563, PubMed : 30898438). Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites (PubMed : 12607005, PubMed : 15201865, PubMed : 16049003, PubMed : 16377563, PubMed : 30898438). Also required for downstream events subsequent to the recruitment of these proteins (PubMed : 12607005, PubMed : 15201865, PubMed : 16049003, PubMed : 16377563, PubMed : 18582474). These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis (PubMed : 12499369, PubMed : 12551934, PubMed : 12607004). ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (PubMed : 12499369, PubMed : 12551934, PubMed : 12607004). Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs) : TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis (PubMed : 30898438). Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs (PubMed : 18411307, PubMed : 18582474, PubMed : 18583988, PubMed : 18678890).

See full target information MDC1

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40049731

2025

MDC1 mediates Pellino recruitment to sites of DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Mònica Torres Esteban,Matthew J Stewart,Eilis Bragginton,Alice Meroni,Annica Pellizzari,Alain Jeanrenaud,Stephen J Smerdon,Manuel Stucki

Exposure and health 16:1039-1052 PubMed39220725

2024

Follicular DNA Damage and Pesticide Exposure Among Latinx Children in Rural and Urban Communities.

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Unspecified application

Species

Unspecified reactive species

Cassandra Lepetit,Mohamed Gaber,Ke Zhou,Haiying Chen,Julia Holmes,Phillip Summers,Kim A Anderson,Richard P Scott,Carey N Pope,Kirstin Hester,Paul J Laurienti,Sara A Quandt,Thomas A Arcury,Pierre-Alexandre Vidi

Nature 621:415-422 PubMed37674080

2023

Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis.

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Unspecified application

Species

Unspecified reactive species

Camille Gelot,Marton Tibor Kovacs,Simona Miron,Emilie Mylne,Alexis Haan,Liza Boeffard-Dosierre,Rania Ghouil,Tatiana Popova,Florent Dingli,Damarys Loew,Josée Guirouilh-Barbat,Elaine Del Nery,Sophie Zinn-Justin,Raphael Ceccaldi

Cell reports 37:110138 PubMed34936865

2021

IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer.

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Unspecified application

Species

Unspecified reactive species

Na-Lee Ka,Ga Young Lim,Sewon Hwang,Seung-Su Kim,Mi-Ock Lee

Cell reports 34:108680 PubMed33503415

2021

Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Yong Lee,Anindya Dutta

Cell death & disease 11:238 PubMed32303682

2020

Biallelic mutations in WRAP53 result in dysfunctional telomeres, Cajal bodies and DNA repair, thereby causing Hoyeraal-Hreidarsson syndrome.

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Unspecified application

Species

Unspecified reactive species

Sofie Bergstrand,Stefanie Böhm,Helena Malmgren,Anna Norberg,Mikael Sundin,Ann Nordgren,Marianne Farnebo

BMC research notes 13:146 PubMed32160908

2020

MDC1 depletion promotes cisplatin induced cell death in cervical cancer cells.

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Unspecified application

Species

Unspecified reactive species

Neeru Singh,Rashmi Bhakuni,Dimple Chhabria,Sivapriya Kirubakaran

Nature communications 11:123 PubMed31913317

2020

Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.

Applications

Unspecified application

Species

Unspecified reactive species

Clémence Mooser,Ioanna-Eleni Symeonidou,Pia-Amata Leimbacher,Alison Ribeiro,Ann-Marie K Shorrocks,Stephanie Jungmichel,Sara C Larsen,Katja Knechtle,Arti Jasrotia,Diana Zurbriggen,Alain Jeanrenaud,Colin Leikauf,Daniel Fink,Michael L Nielsen,Andrew N Blackford,Manuel Stucki

Molecular cell 74:571-583.e8 PubMed30898438

2019

MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.

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Unspecified application

Species

Unspecified reactive species

Pia-Amata Leimbacher,Samuel E Jones,Ann-Marie K Shorrocks,Mara de Marco Zompit,Matthew Day,Jordy Blaauwendraad,Diana Bundschuh,Sarah Bonham,Roman Fischer,Daniel Fink,Benedikt M Kessler,Antony W Oliver,Laurence H Pearl,Andrew N Blackford,Manuel Stucki

Molecular cell 68:61-75.e5 PubMed28943310

2017

ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Yong Lee,Jun-Sub Im,Etsuko Shibata,Anindya Dutta

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