Anti-MDC1 抗体 (ab11171)
Key features and details
- Rabbit polyclonal to MDC1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MDC1 antibody
MDC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MDC1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee -
免疫原
Synthetic peptide corresponding to Human MDC1 aa 1375-1425.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11171の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/5000 - 1/25000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).
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IP |
Use at 2-4 µg/mg of lysate.
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/5000 - 1/25000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa). |
IP
Use at 2-4 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. -
組織特異性
Highly expressed in testis. -
配列類似性
Contains 2 BRCT domains.
Contains 1 FHA domain. -
ドメイン
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX. -
翻訳後修飾
Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
細胞内局在
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. - Information by UniProt
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参照データベース
- Entrez Gene: 9656 Human
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- Unigene: 653495 Human
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別名
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
画像
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All lanes : Anti-MDC1 antibody (ab11171) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 220 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MDC1 with ab11171 at 1/1000 (1µg/ml). Detection: DAB.
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1 mg of HeLa whole cell lysate was incubated with ab11171, 3 µg/mL. For western blotting ab11171, 1 µg/mL was used to confirm successful immunoprecipation.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab11171 は 36 報の論文で使用されています。
- Lin YF et al. Mitotic clustering of pulverized chromosomes from micronuclei. Nature 618:1041-1048 (2023). PubMed: 37165191
- Papathanasiou S et al. Heritable transcriptional defects from aberrations of nuclear architecture. Nature 619:184-192 (2023). PubMed: 37286600
- Trivedi P et al. Mitotic tethering enables inheritance of shattered micronuclear chromosomes. Nature 618:1049-1056 (2023). PubMed: 37316668
- De Marco Zompit M et al. The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis. Nat Commun 13:4143 (2022). PubMed: 35842428
- Li X et al. cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence. Protein Cell 13:47-64 (2022). PubMed: 34676498