AB11169

Anti-MDC1 抗体

Name: Anti-MDC1 抗体 (ab11169) | Abcam
Brand: Abcam Limited
SKU: AB11169
Price: 78000 JPY
Availability: InStock
Rating: 4 (8 reviews)

Anti-MDC1 antibody

(8 Reviews)

(48 Publications)

Rabbit Polyclonal MDC1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 48 publications. Immunogen corresponding to Synthetic Peptide within Human MDC1 aa 450-550.

別名を表示する

KIAA0170, NFBD1, MDC1, Mediator of DNA damage checkpoint protein 1, Nuclear factor with BRCT domains 1

1 Images

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Western blot - Anti-MDC1 antibody (AB11169)

Samples : A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies : A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection : Chemiluminescence with exposure times less than 5 min.

Samples : A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies : A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection : Chemiluminescence with exposure times less than 5 min.

All lanes:

Western blot - Anti-MDC1 antibody (ab11169)

Predicted band size: 227 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human MDC1 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q14676

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

In response to recent feedback about mouse species we no longer guarantee this anymore. Please do contact our scientific support team for more information.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antibodies were affinity purified using the peptide immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MDC1 also known as mediators of DNA damage checkpoint protein 1 or ABF-M 1711 is an important player in the DNA damage response (DDR). It has a mass of approximately 220 kDa. MDC1 is highly expressed in various human tissues particularly where cell turnover is high like in the bone marrow and lymphoid organs. In cellular operations MDC1 serves as a scaffold protein that coordinates recruitment and activation of DDR machinery at sites of double-strand breaks (DSBs) on DNA.

Biological function summary

This protein plays a vital role in maintaining genomic stability by binding to phosphorylated histone H2AX at DSB sites. It is not a solitary player; MDC1 functions as part of a larger protein complex including factors like RNF8 RNF168 and 53BP1. This complex is essential for amplifying the DDR signal and facilitating the repair process through non-homologous end joining (NHEJ) and homologous recombination (HR). MDC1's interaction with other repair proteins helps to extend the signal required for effective DNA repair.

Pathways

MDC1 significantly influences cellular pathways involving DNA damage sensing and repair and cell cycle checkpoints. It ties closely to the ATM kinase pathway which is activated in response to DSBs. MDC1 aids ATM in phosphorylating downstream targets like CHK2 and p53 for cell cycle arrest. It also connects with BRCA1 interacting in HR repair pathways to ensure accurate repair of DSBs. Both ATM and BRCA1 interactions illustrate MDC1's pivotal role in maintaining DNA integrity.

MDC1 expression and functionality have profound implications on cancer and immunodeficiencies. Abnormal MDC1 expression or mutations can lead to impaired DNA repair and genomic instability often associated with tumor progression in cancers such as breast cancer. Moreover MDC1 interacts with proteins like p53 which is a well-known tumor suppressor to hinder cancer development by stopping the cell cycle for repair or triggering apoptosis if the damage is beyond repair. Dysregulation in MDC1-related pathways can also compromise immune system effectiveness further underpinning its significance in disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle (PubMed : 12475977, PubMed : 12499369, PubMed : 12551934, PubMed : 12607003, PubMed : 12607004, PubMed : 12607005, PubMed : 12611903, PubMed : 14695167, PubMed : 15201865, PubMed : 15377652, PubMed : 16049003, PubMed : 16377563, PubMed : 30898438). Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites (PubMed : 12607005, PubMed : 15201865, PubMed : 16049003, PubMed : 16377563, PubMed : 30898438). Also required for downstream events subsequent to the recruitment of these proteins (PubMed : 12607005, PubMed : 15201865, PubMed : 16049003, PubMed : 16377563, PubMed : 18582474). These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis (PubMed : 12499369, PubMed : 12551934, PubMed : 12607004). ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (PubMed : 12499369, PubMed : 12551934, PubMed : 12607004). Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs) : TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis (PubMed : 30898438). Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs (PubMed : 18411307, PubMed : 18582474, PubMed : 18583988, PubMed : 18678890).

See full target information MDC1

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文献 (48)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 129:2014-2024 PubMed37914802

2023

Targeting the COMMD4-H2B protein complex in lung cancer.

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Unspecified application

Species

Unspecified reactive species

Ming Tang,Joshua T Burgess,Mark Fisher,Didier Boucher,Emma Bolderson,Neha S Gandhi,Kenneth J O'Byrne,Derek J Richard,Amila Suraweera

The Journal of biological chemistry 299:105043 PubMed37451480

2023

UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination and DNA repair.

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Unspecified application

Species

Unspecified reactive species

Seong-Ok Lee,Jessica L Kelliher,Wan Song,Kyle Tengler,Aradhan Sarkar,Eloise Dray,Justin W C Leung

Nucleic acids research 50:3922-3943 PubMed35253893

2022

ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination.

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Unspecified application

Species

Unspecified reactive species

Doohyung Lee,Katja Apelt,Seong-Ok Lee,Hsin-Ru Chan,Martijn S Luijsterburg,Justin W C Leung,Kyle M Miller

Oncogene 41:2054-2068 PubMed35177811

2022

LIGHT enhanced bispecific antibody armed T-cells to treat immunotherapy resistant colon cancer.

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Guilin Qiao,Lyonell B Kone,Evan H Phillips,Steve Seung-Young Lee,Grace E Brown,Salman R Khetani,Archana Thakur,Lawrence G Lum,Bellur S Prabhakar,Ajay V Maker

Cell research 32:254-268 PubMed34980897

2022

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

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Unspecified application

Species

Unspecified reactive species

Xiaochen Gai,Di Xin,Duo Wu,Xin Wang,Linlin Chen,Yiqing Wang,Kai Ma,Qilin Li,Peng Li,Xiaochun Yu

Nucleic acids research 49:11666-11689 PubMed34718742

2021

ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition.

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Unspecified application

Species

Unspecified reactive species

Ali Bakr,Joschka Hey,Gianluca Sigismondo,Chun-Shan Liu,Ahmed Sadik,Ashish Goyal,Alice Cross,Ramya Lakshmana Iyer,Patrick Müller,Max Trauernicht,Kersten Breuer,Pavlo Lutsik,Christiane A Opitz,Jeroen Krijgsveld,Dieter Weichenhan,Christoph Plass,Odilia Popanda,Peter Schmezer

Protein & cell 13:47-64 PubMed34676498

2021

cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.

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Unspecified application

Species

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Xiaocui Li,Xiaojuan Li,Chen Xie,Sihui Cai,Mengqiu Li,Heping Jin,Shu Wu,Jun Cui,Haiying Liu,Yong Zhao

Frontiers in cell and developmental biology 9:715126 PubMed34540832

2021

Lysine Methyltransferase Inhibitors Impair H4K20me2 and 53BP1 Foci in Response to DNA Damage in Sarcomas, a Synthetic Lethality Strategy.

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Ignacio Campillo-Marcos,Eva Monte-Serrano,Elena Navarro-Carrasco,Raúl García-González,Pedro A Lazo

Science advances 7: PubMed34452908

2021

Lamin B1 sequesters 53BP1 to control its recruitment to DNA damage.

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Laure Etourneaud,Angela Moussa,Emilie Rass,Diane Genet,Simon Willaume,Caroline Chabance-Okumura,Paul Wanschoor,Julien Picotto,Benoît Thézé,Jordane Dépagne,Xavier Veaute,Eléa Dizet,Didier Busso,Aurélia Barascu,Lamya Irbah,Thierry Kortulewski,Anna Campalans,Catherine Le Chalony,Sophie Zinn-Justin,Ralph Scully,Gaëlle Pennarun,Pascale Bertrand

International journal of molecular sciences 22: PubMed33671470

2021

G-Quadruplex Structures Colocalize with Transcription Factories and Nuclear Speckles Surrounded by Acetylated and Dimethylated Histones H3.

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Unspecified application

Species

Unspecified reactive species

Denisa Komůrková,Alena Svobodová Kovaříková,Eva Bártová

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