JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB4544

Anti-MDA5 抗体

Anti-MDA5 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Goat Polyclonal MDA5 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IFIH1 aa 1-50.

別名を表示する

MDA5, RH116, IFIH1, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicase with 2 CARD domains, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RIG-I-like receptor 2, RNA helicase-DEAD box protein 116, CADM-140 autoantigen, Helicard, MDA-5, RLR-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB4544)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB4544)

ab4544 (10ug/ml) staining human MDA5 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

ab4544 shows strong cytoplasmic staining of some cells in the islets of Langerhans.

Flow Cytometry - Anti-MDA5 antibody (AB4544)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MDA5 antibody (AB4544)

Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488® secondary antibody (1 μg/ml).

IgG control : Non-immunized goat IgG (black line) followed by Alexa Fluor 488® secondary antibody.

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt, IHC-P

applications

免疫原

Synthetic Peptide within Human IFIH1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BYX4

style
left-column

代替製品

Primary Antibodies

AB126630

Anti-MDA5 antibody [EPR6743]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-MDA5 antibody [EPR6743] (AB126630)

  • 5
  • 2
Primary Antibodies

AB79055

Anti-MDA5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)

  • 4
  • 1
Primary Antibodies

AB219952

Anti-MDA5 antibody [EPR6743] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-MDA5 antibody [EPR6743] - BSA and Azide free (AB219952)

  • 0
  • 0

組合せ製品

Assay Kits

AB309325

Human MDA5 ELISA Kit

Sandwich ELISA - Human MDA5 ELISA Kit (AB309325)

  • 0
  • 0
Primary Antibodies

AB238254

Anti-RIG-I/DDX58 antibody

Flow Cytometry (Intracellular) - Anti-RIG-I/DDX58 antibody (AB238254)

  • 0
  • 0
Primary Antibodies

AB284603

Anti-MX1 antibody [EPR24485-19]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-MX1 antibody [EPR24485-19] (AB284603)

  • 3
  • 1
Primary Antibodies

AB285367

Anti-ISG15 antibody [EPR24482-49]

BOND RX™ Validated|RabMAb|20ul selling size|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody [EPR24482-49] (AB285367)

  • 0
  • 0
Primary Antibodies

AB238675

Anti-Interferon beta antibody [C5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon beta antibody [C5] (AB238675)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MDA5 also known as melanoma differentiation-associated protein 5 or IFIH1 is a cytosolic sensor involved in the detection of viral RNA. It is a member of the RIG-I-like receptor family and possesses a molecular weight of approximately 116 kilodaltons. MDA5 protein is expressed across various tissues but shows higher expression in the immune system's cells including macrophages and dendritic cells. MDA5 recognizes long double-stranded RNA a common viral replication byproduct triggering immune responses.
Biological function summary

In cellular defense mechanisms MDA5 plays an important role in initiating antiviral responses. It operates as part of a signaling complex that includes MAVS (mitochondrial antiviral-signaling protein) leading to the production of type I interferons and pro-inflammatory cytokines. These responses promote an antiviral state in host cells aiding in the containment and clearance of viral infections. MDA5 antibodies can be useful tools for studying these immune processes.

Pathways

MDA5 interacts with antiviral signaling pathways such as the IFN signaling pathway and the RIG-I-like receptor signaling pathway. Upon recognizing viral RNA MDA-5 cooperates with proteins like RIG-I and MAVS to activate downstream signaling cascades that mediate immune responses. This collaboration enables efficient viral recognition and response limiting viral replication and spread.

MDA5 has associations with autoimmune diseases like systemic lupus erythematosus and certain types of dermatomyositis. These conditions often involve autoantibodies against MDA5 indicating a possible role in pathogenesis. In these diseases altered function or regulation of related proteins including IFN-induced proteins may contribute to chronic inflammation and autoimmunity. Understanding MDA5's involvement in these contexts is essential for developing therapeutic strategies.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines (PubMed : 28594402, PubMed : 32169843, PubMed : 33727702). Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length) (PubMed : 22160685). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases : TBK1 and IKBKE which phosphorylate interferon regulatory factors : IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus (PubMed : 28606988). Detects coronavirus SARS-CoV-2 (PubMed : 33440148, PubMed : 33514628). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
See full target information IFIH1
style
left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 13:881997 PubMed35957810

2022

Detection of Antiviral Tissue Responses and Increased Cell Stress in the Pancreatic Islets of Newly Diagnosed Type 1 Diabetes Patients: Results From the DiViD Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Krogvold,Pia Leete,Ida M Mynarek,Mark A Russell,Ivan C Gerling,Nataliya I Lenchik,Clayton Mathews,Sarah J Richardson,Noel G Morgan,Knut Dahl-Jørgensen

Frontiers in immunology 13:833141 PubMed35359976

2022

Increased Expression of Viral Sensor MDA5 in Pancreatic Islets and in Hormone-Negative Endocrine Cells in Recent Onset Type 1 Diabetic Donors.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Nigi,Noemi Brusco,Giuseppina E Grieco,Daniela Fignani,Giada Licata,Caterina Formichi,Elena Aiello,Lorella Marselli,Piero Marchetti,Lars Krogvold,Knut Dahl Jorgensen,Guido Sebastiani,Francesco Dotta

PLoS pathogens 17:e1009305 PubMed33556144

2021

Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Natallia Mikhalkevich,Ina P O'Carroll,Rok Tkavc,Kateryna Lund,Gauthaman Sukumar,Clifton L Dalgard,Kory R Johnson,Wenxue Li,Tongguang Wang,Avindra Nath,Sergey Iordanskiy

Arthritis and rheumatism 60:2193-200 PubMed19565506

2009

RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease.

Applications

WB

Species

Unspecified reactive species

Shinji Sato,Kana Hoshino,Takashi Satoh,Tomonobu Fujita,Yutaka Kawakami,Takashi Fujita,Masataka Kuwana
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com