AB102678

Anti-MCTS1/MCT-1 抗体

Name: Anti-MCTS1/MCT-1 抗体 (ab102678) | Abcam
Brand: Abcam Limited
SKU: AB102678
Price: 76000 JPY
Availability: InStock

Anti-MCTS1/MCT-1 antibody

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(2 Publications)

Rabbit Polyclonal MCTS1/MCT-1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCTS1 aa 1-200.

別名を表示する

MCT1, MCTS1, Malignant T-cell-amplified sequence 1, MCT-1, Multiple copies T-cell malignancies

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue stained for MCTS1 protein at cytoplasm with ab102678 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue stained for MCTS1 protein at cytoplasm with ab102678 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-MCTS1/MCT-1 antibody (AB102678)

12% SDS-PAGE

All lanes:

Western blot - Anti-MCTS1/MCT-1 antibody (ab102678) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 21 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MCTS1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9ULC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MCTS1 also known as malignancy-associated gene protein MCT-1 is a protein with a molecular weight of about 18 kDa. It functions as a translation enhancer and is involved in the regulation of protein synthesis. The protein can bind to the eukaryotic translation initiation factor 3 (eIF3) and is expressed in a variety of tissues with higher expression levels often observed in tumor cells. MCTS1's role in promoting efficient translation initiation highlights its mechanistic involvement in the cellular growth process.

Biological function summary

This protein influences cell cycle progression and survival. It forms part of a complex with density-regulated protein (DENR) which aids in ribosomal recycling and the translation initiation of short upstream open reading frames. MCTS1 contributes to the modulation of cellular response to stress and is significant in processes related to cancer cell proliferation.

Pathways

The role of MCTS1 includes involvement in the mTOR and MAPK signaling pathways which regulate cell growth and survival. MCTS1 interacts with the mTOR signaling pathway and can influence translation machinery components linking it to growth-related processes. Additionally its association with eIF3 positions it within the context of translation initiation pathways where it cooperates with other proteins to fine-tune protein synthesis.

The overexpression of MCTS1 links it to cancers such as breast cancer and non-small cell lung cancer. Research indicates that MCTS1 alongside proteins like mTOR and eIF3 play a role in tumor progression and oncogenesis. The protein's involvement in translation initiation and cell growth processes suggests potential as a biomarker and target for therapeutic interventions in these malignancies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Translation regulator forming a complex with DENR to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation (PubMed : 16982740, PubMed : 20713520, PubMed : 37875108). This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent (PubMed : 16982740, PubMed : 20713520, PubMed : 37875108). Consequently, modulates various unrelated biological processes including cell cycle regulation and DNA damage signaling and repair (PubMed : 10440924, PubMed : 11709712, PubMed : 12637315, PubMed : 15897892, PubMed : 16322206, PubMed : 17016429, PubMed : 17416211, PubMed : 9766643). Notably, it positively regulates interferon gamma immunity to mycobacteria by enhancing the translation of JAK2 (PubMed : 37875108).

See full target information MCTS1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 22:531 PubMed34079590

2021

MCTS1 promotes the development of lung adenocarcinoma by regulating E2F1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cun Gao,Rui Dong,Yongmeng Li,Jinghui Liang,Hui Tian

Proteomics. Clinical applications 8:130-42 PubMed24458570

2014

Aberrant glycosylation in the human trabecular meshwork.

Applications

Unspecified application

Species

Unspecified reactive species

Adam E Sienkiewicz,Brandon N Rosenberg,Genea Edwards,Teresia A Carreon,Sanjoy K Bhattacharya

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCTS1/MCT-1 抗体

Anti-MCTS1/MCT-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Western blot - Anti-MCTS1/MCT-1 antibody (AB102678)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise