Anti-MCM6 抗体 (ab184147)
Key features and details
- Rabbit polyclonal to MCM6
- Suitable for: WB, ICC/IF, IHC-P, ChIP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM6 antibody
MCM6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, ChIPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rhesus monkey
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免疫原
Recombinant fragment within Human MCM6 aa 682-821 (C terminal). The exact sequence is proprietary.
Database link: Q14566 -
ポジティブ･コントロール
- 293T, A431, HeLa or HepG2 lysate, Human ovarian carcinoma or gastric carcinoma tissue, U2OS cells, HeLa chromatin extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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| ICC/IF |
1/1000.
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| IHC-P |
1/100 - 1/1000.
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| ChIP |
Use at an assay dependent concentration.
5ug |
| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
|
ICC/IF
1/1000. |
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IHC-P
1/100 - 1/1000. |
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ChIP
Use at an assay dependent concentration. 5ug |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Binds to chromatin during G1 and detach from it during S phase. - Information by UniProt
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参照データベース
- Entrez Gene: 4175 Human
- Omim: 601806 Human
- SwissProt: Q14566 Human
- Unigene: 444118 Human
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別名
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
see all
画像
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Western blot - Anti-MCM6 antibody (ab184147)All lanes : Anti-MCM6 antibody (ab184147) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : A431 lysate
Lane 3 : HeLa lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 93 kDa7.5 % SDS-PAGE
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ChIP - Anti-MCM6 antibody (ab184147)Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either normal rabbit IgG (1) or ab184147 (2). The precipitated DNA was detected by PCR with primer set targeting to Myc promoter (1 and 2).
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Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody (ab184147)Immunofluorescence analysis of 4% paraformaldehyde fixed U2OS cells, labeling MCM6 with ab184147 at a 1/500 dilution (left image), and costained with Hoescht 33342 (right image).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab184147)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab184147)Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling MCM6 with ab184147 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab184147 は 1 報の論文で使用されています。
- Lee JS & Zhang Z O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence. Proc Natl Acad Sci U S A 113:E3213-20 (2016). PubMed: 27217568