Anti-MCM5 抗体 (ab17967)
Key features and details
- Rabbit polyclonal to MCM5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM5 antibody
MCM5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Orangutan
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免疫原
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17967の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa).
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Detects a band of approximately 97 kDa (predicted molecular weight: 82 kDa). |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4174 Human
- Omim: 602696 Human
- SwissProt: P33992 Human
- Unigene: 517582 Human
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別名
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
画像
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Western blot - Anti-MCM5 antibody (ab17967)All lanes : Anti-MCM5 antibody (ab17967) at 0.05 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 82 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab17967 は 26 報の論文で使用されています。
- Ferreira P et al. Refining the domain architecture model of the replication origin firing factor Treslin/TICRR. Life Sci Alliance 5:N/A (2022). PubMed: 35091422
- Zhao Y et al. A Combined Treatment with Berberine and Andrographis Exhibits Enhanced Anti-Cancer Activity through Suppression of DNA Replication in Colorectal Cancer. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35337060
- Wang Y et al. LSD1 is required for euchromatic origin firing and replication timing. Signal Transduct Target Ther 7:102 (2022). PubMed: 35414135
- Polasek-Sedlackova H et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nat Commun 13:6090 (2022). PubMed: 36241664
- Giles KA et al. BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer. Clin Epigenetics 13:37 (2021). PubMed: 33596994