Name: Anti-MCM2 (phospho S53) antibody [EP4120]
Brand: RabMAb
SKU: ab109133
Rating: 5 (4 reviews)

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Western blot - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4120] to MCM2 (phospho S53)
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品名

Anti-MCM2 (phospho S53) antibody [EP4120]
MCM2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP4120] to MCM2 (phospho S53)
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa whole cell lysate (ab150035). IHC-P:Human tonsil tissue. ICC/IF: HeLa cells.
特記事項
This product has switched from a hybridoma to recombinant production method on 9th June 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP4120
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109133の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/20.
WB	(1)	1/1000.
IHC-P	(2)	1/2400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF	(1)	1/50.

特記事項
Flow Cyt (Intra) 1/20.
WB 1/1000.
IHC-P 1/2400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50.

追加情報

Is unsuitable for Flow Cyt or IP.

機能

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
配列類似性

Belongs to the MCM family.
Contains 1 MCM domain.
翻訳後修飾

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
細胞内局在

Nucleus.
Target information above from: UniProt accession P49736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
- CDC like 1 antibody
- cdc19 antibody
- CDCL 1 antibody
- CDCL1 antibody
- Cell devision cycle like 1 antibody
- Cyclin like 1 antibody
- D3S3194 antibody
- DNA replication licensing factor MCM2 antibody
- KIAA0030 antibody
- MCM 2 antibody
- MCM2 antibody
- MCM2 minichromosome maintenance deficient 2 mitotin antibody
- MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
- MCM2 minichromosome maintenance deficient 2, mitotin antibody
- MCM2_HUMAN antibody
- MGC10606 antibody
- Minichromosome maintenance complex component 2 antibody
- Minichromosome maintenance deficient 2 (mitotin) antibody
- Minichromosome maintenance deficient 2 mitotin antibody
- Minichromosome maintenance protein 2 antibody
- Minichromosome maintenance protein 2 homolog antibody
- Mitotin antibody
- Nuclear protein BM28 antibody
- OTTHUMP00000216047 antibody
- OTTHUMP00000216050 antibody
see all

Western blot - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133) at 1/10000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 130 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133) at 1/10000 dilution

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2 : NIH/3T3 whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 130 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

All lanes : Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133) at 1/10000 dilution

Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2 : C6 whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 130 kDa why is the actual band size different from the predicted?

Exposure time: 60 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa cells labelling MCM2 with ab109133 at 1/50 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling MCM2 with ab109133 at 1/50 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of HeLa cells. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MCM2 with ab109133 at 1/2400 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human tonsil tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MCM2 with ab109133 at 1/2400 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human colon tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling MCM2 with ab109133 at 1/2400 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse spleen tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling MCM2 with ab109133 at 1/2400 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat spleen tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
Anti-MCM2 (phospho S53) antibody [EP4120] (ab109133)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab109133 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109133 は 9 報の論文で使用されています。

Borel V et al. Disrupted control of origin activation compromises genome integrity upon destabilization of Polε and dysfunction of the TRP53-CDKN1A/P21 axis. Cell Rep 39:110871 (2022). PubMed: 35649380
Guo Y et al. Targeting CDC7 potentiates ATR-CHK1 signaling inhibition through induction of DNA replication stress in liver cancer. Genome Med 13:166 (2021). PubMed: 34663432
Wienert B et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun 11:2109 (2020). PubMed: 32355159
Guarino Almeida E et al. A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation. Nucleic Acids Res 48:7844-7855 (2020). PubMed: 32652013
Wang C et al. Inducing and exploiting vulnerabilities for the treatment of liver cancer. Nature 574:268-272 (2019). PubMed: 31578521
Qian X et al. PTEN Suppresses Glycolysis by Dephosphorylating and Inhibiting Autophosphorylated PGK1. Mol Cell 76:516-527.e7 (2019). PubMed: 31492635
Li X et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930
Alver RC et al. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep 18:2508-2520 (2017). PubMed: 28273463
Garzón J et al. Shortage of dNTPs underlies altered replication dynamics and DNA breakage in the absence of the APC/C cofactor Cdh1. Oncogene 36:5808-5818 (2017). PubMed: 28604743

レビューと Q&A

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レビューを送る質問を送る

1-4 of 4 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Human Dermal Fibroblasts)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Human Dermal Fibroblasts

Blocking step

10% FBS, 1% BSA, 0.1% Triton X-100 in PBS as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 11% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 08 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

colon

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 31 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

colon

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 31 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293T)

Loading amount

50 µg

Specification

HEK293T

Treatment

Cdc7 RNAi

Gel Running Conditions

Reduced Denaturing (8% gel)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 27 2012

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (9)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-MCM2 (phospho S53) antibody [EP4120]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM2 (phospho S53) antibody [EP4120]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM2 (phospho S53) antibody [EP4120]

Western blot abreview for Anti-MCM2 (phospho S53) antibody [EP4120]