Anti-MCM2 (phospho S53) 抗体 (ab70367)
Key features and details
- Rabbit polyclonal to MCM2 (phospho S53)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MCM2 (phospho S53) antibody
MCM2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM2 (phospho S53) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human MCM2 (phospho S53).
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ポジティブ･コントロール
- Whole cell lysate from asynchronous 293T cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70367の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/2500. Detects a band of approximately 117 kDa (predicted molecular weight: 100 kDa).
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/2500. Detects a band of approximately 117 kDa (predicted molecular weight: 100 kDa). |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Entrez Gene: 192338 Zebrafish
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
see all -
別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S53) antibody (ab70367)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM2 (phospho S53) with ab70367 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-MCM2 (phospho S53) antibody (ab70367)All lanes : Anti-MCM2 (phospho S53) antibody (ab70367) at 0.1 µg/ml
Lane 1 : Whole cell lysate from asynchronous 293T cells
Lane 2 : Whole cell lysate from asynchronous 293T cells treated with a phosphatase.
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
The bottom panel shows total MCM2 staining; the blot was stripped and then blotted with rabbit anti-total MCM2 antibody at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 3 minutes (upper panel) and 10 seconds (lower panel).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70367 は 3 報の論文で使用されています。
- Almeida R et al. Chromatin conformation regulates the coordination between DNA replication and transcription. Nat Commun 9:1590 (2018). PubMed: 29686321
- Rodriguez-Acebes S et al. Uncoupling fork speed and origin activity to identify the primary cause of replicative stress phenotypes. J Biol Chem 293:12855-12861 (2018). PubMed: 29959228
- Liu H et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB ; Human . PubMed: 20818375