Anti-MCM2 (phospho S40 + S41) 抗体 (ab84391)
Key features and details
- Rabbit polyclonal to MCM2 (phospho S40 + S41)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM2 (phospho S40 + S41) antibody
MCM2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM2 (phospho S40 + S41) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human MCM2 (phospho S40 + S41).
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ポジティブ・コントロール
- Human colon adenocarcinoma and mouse squamous cell carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab84391 is affinity purified using an epitope specific to MCM2 (phospho S40 + S41) immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84391の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
特記事項 |
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Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Entrez Gene: 448458 Xenopus tropicalis
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- SwissProt: Q6DIH3 Xenopus tropicalis
see all -
別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
画像
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ab84391, at a 1/250 dilution, staining MCM2 (phospho S40 + S41) in formalin fixed, paraffin embedded human colon adenocarcinoma tissue by Immunohistochemistry.
1) Mock phosphatase treated section
2) Calf intestinal phosphatase-treated section
Detection: DAB staining. -
ab84391, at a 1/250 dilution, staining MCM2 (phospho S40 + S41) in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue by Immunohistochemistry.
1) Mock phosphatase treated section
2) Calf intestinal phosphatase-treated section
Detection: DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84391 は論文での使用が確認できていません。