Anti-MCM2 (phospho S139) 抗体 [EPR10819] (ab170873)
![Western blot - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-171038-anti-mcm2-phospho-s139-antibody-epr10819-western-blot.jpg "Western blot - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10819] to MCM2 (phospho S139)
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MCM2 (phospho S139) antibody [EPR10819]
MCM2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10819] to MCM2 (phospho S139) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10819 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170873の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/10 - 1/100. |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
画像
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Western blot - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)All lanes : Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Hela cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 102 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-221475-anti-mcm2-phospho-s139-antibody-epr10819-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)ab170873 showing +ve staining in Human normal colon tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-221474-anti-mcm2-phospho-s139-antibody-epr10819-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)ab170873 showing +ve staining in Human glioma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-171040-anti-mcm2-phospho-s139-antibody-epr10819-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM2 using ab170873 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-171039-anti-mcm2-phospho-s139-antibody-epr10819-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling MCM2 using ab170873 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-171041-anti-mcm2-phospho-s139-antibody-epr10819-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)Western blot analysis on immunoprecipitation pellet from Jurkat lysate using ab170873 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873) at 1/10 dilution + Jurkat cell lysate at 10 µg -
![Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)](https://www.abcam.co.jp/ps/products/170/ab170873/Images/ab170873-9-benefits-of-recombinant-antibodies.png)
Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab170873 は論文での使用が確認できていません。