AB6153

Anti-MCM2 抗体 [CRCT2.1 (D1.9H5)]

Name: Anti-MCM2 抗体 [CRCT2.1 (D1.9H5)] (ab6153) | Abcam
Brand: Abcam Limited
SKU: AB6153
Price: 81000 JPY
Availability: InStock
Rating: 5 (7 reviews)

Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]

(7 Reviews)

(10 Publications)

Mouse Monoclonal MCM2 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCM2.

別名を表示する

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

1 Images

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Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (AB6153)

Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

All lanes:

Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)

Predicted band size: 102 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CRCT2.1 (D1.9H5)

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Recombinant Full Length Protein corresponding to Human MCM2.

P49736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).

See full target information MCM2

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:2109 PubMed32355159

2020

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair.

Applications

Unspecified application

Species

Unspecified reactive species

Beeke Wienert,David N Nguyen,Alexis Guenther,Sharon J Feng,Melissa N Locke,Stacia K Wyman,Jiyung Shin,Katelynn R Kazane,Georgia L Gregory,Matthew A M Carter,Francis Wright,Bruce R Conklin,Alex Marson,Chris D Richardson,Jacob E Corn

Cell 174:803-817.e16 PubMed30057114

2018

Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications.

Applications

Unspecified application

Species

Unspecified reactive species

Sweta Mishra,Capucine Van Rechem,Sangita Pal,Thomas L Clarke,Damayanti Chakraborty,Sarah D Mahan,Joshua C Black,Sedona E Murphy,Michael S Lawrence,Danette L Daniels,Johnathan R Whetstine

Oncology letters 13:1891-1898 PubMed28454340

2017

Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma.

Applications

IHC-P

Species

Human

Cristovam Scapulatempo-Neto,Carlos Veo,José Humberto T G Fregnani,Adriana Lorenzi,Allini Mafra,Armando G F Melani,Edgar Antonio Alemán Loaiza,Luciana Albina Reis Rosa,Cristina Mendes de Oliveira,José Eduardo Levi,Adhemar Longatto-Filho

Journal of cell science 124:2058-69 PubMed21610089

2011

Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Tianbu Zhang,Alexander R Langley,Christo P Christov,Eyemen Kheir,Thomas Shafee,Timothy J Gardiner,Torsten Krude

Proceedings of the National Academy of Sciences of the United States of America 107:7377-82 PubMed20368428

2010

Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing.

Applications

Species

Human

Michael Shtutman,Anil Maliyekkel,Yu Shao,C Steven Carmack,Mirza Baig,Natalie Warholic,Kelly Cole,Eugenia V Broude,Timothy T Harkins,Ye Ding,Igor B Roninson

BMC microbiology 8:133 PubMed18680610

2008

Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection.

Applications

IHC-P

Species

Human

Saira Sarfraz,Saeed Hamid,Anwar Siddiqui,Snawar Hussain,Shahid Pervez,Graeme Alexander

The Journal of cell biology 177:87-101 PubMed17420292

2007

Evidence for a stem cell hierarchy in the adult human breast.

Applications

Unspecified application

Species

Unspecified reactive species

René Villadsen,Agla J Fridriksdottir,Lone Rønnov-Jessen,Thorarinn Gudjonsson,Fritz Rank,Mark A LaBarge,Mina J Bissell,Ole W Petersen

Human reproduction (Oxford, England) 22:1778-88 PubMed17371803

2007

Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea L Niklaus,Mira Aubuchon,Gregory Zapantis,Ping Li,Hong Qian,Barbara Isaac,Mimi Y Kim,Goli Adel,Jeffrey W Pollard,Nanette F Santoro

The Journal of biological chemistry 281:35942-53 PubMed17001081

2006

Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A.

Applications

Species

Human

Núria Canela,Mar Orzáez,Raquel Fucho,Francesca Mateo,Ricardo Gutierrez,Antonio Pineda-Lucena,Oriol Bachs,Enrique Pérez-Payá

Molecular and cellular biology 25:6937-47 PubMed16055707

2005

p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation.

Applications

Species

Unspecified reactive species

Anja Duursma,Reuven Agami

View all publications

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Abcam product promise

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Anti-MCM2 抗体 [CRCT2.1 (D1.9H5)]

Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]

Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (AB6153)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (10)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise